別品名 |
CD340, cluster of differentiation 340, ERBB2 (human), human epidermal growth factor receptor 2, HER2/neu, recombinant Monoclonal HER2, RabMAb HER2, Trastuzumab, Herceptin, Humanized Antibody
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種由来 |
Human
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標識物 |
Unlabeled
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Flow Cytometry
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抗体クラス |
IgG
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交差種 |
Human
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GENE ID |
2064
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Accession No.(Gene/Protein) |
NP_001005862, P04626
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形状 |
液状
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※サムネイル画像をクリックすると拡大画像が表示されます。
Flow Cytometry of Humanized Recombinant Anti human HER2 Fab fragment Antibody. Primary Antibody: Recombinant Anti HER2 Fab fragment (red line) recognizes HER2 on SK OV 3 cells while using Anti human kappa FITC secondary antibody at 0.2 uL with a 56.8% positive cells. Controls used: (black line) no primary antibody, no secondary antibody, 0.6%; (green line) no primary antibody, Anti human kappa FITC secondary antibody at 0.2 uL, 1.0%.
SDS-PAGE of Humanized Recombinant Anti-human HER2 Fab fragment Antibody. Lane 1: Molecular Weight Ladder. Lane 2: reduced recombinant HER2 Fab fragment. Lane 3: non-reduced recombinant HER2 Fab fragment.
ELISA results show the sensitivity of Humanized Recombinant Anti-human HER2 Fab fragment Antibody tested against purified HER2 protein. Each well was coated in duplicate with 1.0 μg of HER2. The starting dilution of antibody was 5.0 μg/ml.
Western Blot of Humanized Recombinant Anti-human HER2 Fab fragment Antibody. Lane 1 and 8: Molecular Weight Marker. Lane 2 and 6: 0.02ug HER2. Lane 3 and 7: 20ug non-spiked SKOV-3 lysate. Lane 4 and 9: 20ug SKOV-3 lysate spiked with 0.05ug HER2. Lane 5 and 10: 20ug SKOV-3 lysate spiked with 0.02ug HER2. Primary Antibody: Anti-HER2 Fab incubated with lanes 1-5 only. Secondatry Antibody: Anti-F(ab)'2 HRP conjugate at 1:5000. Anti-HER2 Fab antibody observed in native HER2 and spiked HER2 in SKOV-3 cell lysates.
Western Blot of Humanized Recombinant Anti-human HER2 Fab fragment Antibody. Lane 1: Molecular Weight Ladder. Lane 2, 5, and 8: Empty. Lane 3, 6, and 9: HER2 reduced. Lane 4, 7, 10: HER2 non-reduced. Protein Load: Lane 3-4: 0.5ug. Lane 6-7: 0.1ug. Lane 9-10: 0.02ug. Primary Antibody: anti-HER2 Fab at 1:2000 for 2 hours at 4 C. Secondary Antibody: Anti-human kappa FITC conjugate at 1:1000 for 2 hours at 4C. Exposure time 60s.
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Flow Cytometry of Humanized Recombinant Anti human HER2 Fab fragment Antibody. Primary Antibody: Recombinant Anti HER2 Fab fragment (red line) recognizes HER2 on SK OV 3 cells while using Anti human kappa FITC secondary antibody at 0.2 uL with a 56.8% positive cells. Controls used: (black line) no primary antibody, no secondary antibody, 0.6%; (green line) no primary antibody, Anti human kappa FITC secondary antibody at 0.2 uL, 1.0%.
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メーカー |
品番 |
包装 |
RKL
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400-001-GY3
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50 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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