適用 |
IHC paraffin embedding section Enzyme Linked Immunosorbent Assay Immunocytochemistry (cell) Dot Blot Methylated DNA Immunoprecipitation
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免疫動物 |
Rabbit Mono
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抗体クラス |
IgG
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クローン |
RM236
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形状 |
液状
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Hydroxymethylated DNA immunoprecipitation (hMeDIP) analysis of 5-hydroxymethylcytosine (5-hmC) monoclonal antibody, clone RM236 (Cat # MAB15116) at a 10:1 DNA:Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1 ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain with 5-hydroxymethylcytosine (5-hmC) monoclonal antibody, clone RM236 (Cat # MAB15116).
Immunocytochemistry staining of HeLa cells with 5-hydroxymethylcytosine (5-hmC) monoclonal antibody, clone RM236 (Cat # MAB15116) (Red). Actin filaments was labeled with fluorescein phalloidin (Green).
ELISA analysis of single stranded DNA with 5-hydroxymethylcytosine (5-hmC) monoclonal antibody, clone RM236 (Cat # MAB15116). The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of MAB15116 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Dot blot analysis of double stranded DNA with 5-hydroxymethylcytosine (5-hmC) monoclonal antibody, clone RM236 (Cat # MAB15116). The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with MAB15116.
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Hydroxymethylated DNA immunoprecipitation (hMeDIP) analysis of 5-hydroxymethylcytosine (5-hmC) monoclonal antibody, clone RM236 (Cat # MAB15116) at a 10:1 DNA:Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1 ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.
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メーカー |
品番 |
包装 |
ABV
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MAB15116
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50 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
1週間程度
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保存温度 |
-20℃
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