別品名 |
Transcription factor A, mitochondrial, mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM, TCF6, TCF6L2
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抗原部位 |
a.a.216-246
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Ig fraction - Protein A
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適用 |
Western Blot IHC paraffin embedding section Immuno Fluorescence Flow Cytometry
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免疫動物 |
Rabbit
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抗体クラス |
Ig
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交差種 |
Human
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GENE ID |
7019
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Accession No.(Gene/Protein) |
Q00059
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Gene Symbol |
TFAM
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形状 |
液状
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その他 |
[Protein GI Number]417324 [Swiss-Prot No]Q00059
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参考文献 |
Wang, W., et al. Nucleic Acids Res. (2010) In press : Corneveaux, J.J., et al. Hum. Mol. Genet. 19(16):3295-3301(2010) Akhmetov, I.I., et al. Fiziol Cheloveka 36(2):121-125(2010) Shulman, J.M., et al. PLoS ONE(6), E11244 (2010) :
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Western blot analysis of lysates from Hela, HepG2, U-2OS cell line (from left to right), using TFAM Antibody at 1:1000 at each lane.
Western blot analysis of lysate from 293T cell line, using TFAM Antibody at 1:1000.
TFAM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fluorescent confocal image of NCI-H460 cell stained with TFAM Antibody . NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAM primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TFAM immunoreactivity is localized to mitochondrion significantly.
Western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).
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Western blot analysis of lysates from Hela, HepG2, U-2OS cell line (from left to right), using TFAM Antibody at 1:1000 at each lane.
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メーカー |
品番 |
包装 |
PSC
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55-117
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400 UL [0.5 mg/ml]
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※表示価格について
当社在庫 |
なし
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納期目安 |
3週間程度
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保存温度 |
-20℃
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