別品名 |
CYGB | Cytoglobin | Hgb | Histoglobin | Q8WWM9
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抗原部位 |
a.a.1-190
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot IHC paraffin embedding section
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse Rat
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GENE ID |
114757
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Accession No.(Gene/Protein) |
Q8WWM9
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Gene Symbol |
CYGB
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分子量 |
21405 MW
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形状 |
凍結乾燥品
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参考文献 |
1. Asahina, K., Kawada, N., Kristensen, D. B., Nakatani, K., Seki, S., Shiokawa, M., Tateno, C., Obara, M., Yoshizato, K. Characterization of human stellate cell activation-associated protein and its expression in human liver. Biochim. Biophys. Acta 1577: 471-475, 2002. 2. Burmester, T., Ebner, B., Weich, B., Hankeln, T. Cytoglobin: a novel globin type ubiquitously expressed in vertebrate tissues. Molec. Biol. Evol. 19: 416-421, 2002. 3. Geuens, E., Brouns, I., Flamez, D., Dewilde, S., Timmermans, J.-P., Moens, L. A globin in the nucleus! J. Biol. Chem. 278: 30417-30420, 2003.
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Western blot analysis of Cytoglobin using anti-Cytoglobin antibody (A01197). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytoglobin antigen affinity purified polyclonal antibody (Catalog # A01197) at 0.5 I?g/mL overnight at 4A°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytoglobin at approximately 21KD. The expected band size for Cytoglobin is at 21KD.
IHC analysis of Cytoglobin using anti- Cytoglobin antibody (A01197). Cytoglobin was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I?g/ml rabbit anti- Cytoglobin Antibody (A01197) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Cytoglobin using anti- Cytoglobin antibody (A01197). Cytoglobin was detected in paraffin-embedded section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I?g/ml rabbit anti- Cytoglobin Antibody (A01197) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Cytoglobin using anti- Cytoglobin antibody (A01197). Cytoglobin was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I?g/ml rabbit anti- Cytoglobin Antibody (A01197) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Cytoglobin using anti- Cytoglobin antibody (A01197). Cytoglobin was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I?g/ml rabbit anti- Cytoglobin Antibody (A01197) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Cytoglobin using anti- Cytoglobin antibody (A01197). Cytoglobin was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I?g/ml rabbit anti- Cytoglobin Antibody (A01197) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of Cytoglobin using anti-Cytoglobin antibody (A01197). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytoglobin antigen affinity purified polyclonal antibody (Catalog # A01197) at 0.5 I?g/mL overnight at 4A°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytoglobin at approximately 21KD. The expected band size for Cytoglobin is at 21KD.
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メーカー |
品番 |
包装 |
BBT
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A01197
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
3週間程度
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法規制 |
毒
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保存温度 |
-20℃
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