別品名 |
Gli-2, Gli 2, Gli2, zinc finger protein GLI2, Tax helper protein, Thp
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抗原部位 |
internal region
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種由来 |
Mouse
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Mouse
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Accession No.(Gene/Protein) |
Q8K0K3
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Gene Symbol |
GLI2
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形状 |
滅菌済み液状品
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※サムネイル画像をクリックすると拡大画像が表示されます。
Rocklands Affinity Purified anti-mouse Gli-2 antibody was used at 10
Rocklands Affinity Purified anti-mouse Gli-2 antibody was used at 10
Figure 2. Western blot analysis of GLI2 using anti-GLI2 antibody (A00701). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (Catalog # A00701) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for GLI2.
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Rocklands Affinity Purified anti-mouse Gli-2 antibody was used at 10
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メーカー |
品番 |
包装 |
BBT
|
A00701
|
100 UG [1.02 mg/ml]
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※表示価格について
当社在庫 |
なし
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納期目安 |
1週間程度
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保存温度 |
-20℃
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