別品名 |
APG6 antibody; ATG 6 antibody; ATG6 antibody; ATG6 autophagy related 6 homolog antibody; Bcl-2-interacting protein beclin antibody; Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody; Beclin 1 autophagy related antibody; Beclin-1 antibody; Beclin1 antibody; BECN 1 antibody; Becn1 antibody; BECN1_HUMAN antibody; Coiled coil myosin like BCL2 interacting protein antibody; Coiled-coil myosin-like BCL2-interacting protein antibody; GT197 antibody; Protein GT197 antibody; VPS 30 antibody; VPS30 antibody
|
抗原部位 |
a.a.1-100
|
種由来 |
Human
|
標識物 |
Unlabeled
|
精製度 |
Ig fraction - Protein G
|
適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry Immuno Fluorescence
|
免疫動物 |
Rabbit
|
抗体クラス |
IgG
|
交差種 |
Human Mouse
|
Accession No.(Gene/Protein) |
Q14457
|
形状 |
液状
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, SH-SY5Y whole cell lysate All lanes: BECN1 antibody at 4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa
IHC image of CSB-PA617917LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-PA617917LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
|
|
Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, SH-SY5Y whole cell lysate All lanes: BECN1 antibody at 4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa
|
|
|
メーカー |
品番 |
包装 |
CSB
|
CSB-PA617917LA01HU
|
100 UG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
2週間程度
|
保存温度 |
-20℃
|
|