適用 |
Flow Cytometry
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使用目的 |
デュアルカラーフローサイトメトリによってVASPリン酸化部位を通したP2Y12レセプター反応性を測定するためのキットです。
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構成内容 |
・Diluent ・PGE1 ・PGE1 + ADP ・fixative ・Anti VASP-P mouse monoclonal antibody+permeabilization agent ・negative isotypic control+ permeabilization agent ・Anti mouse IgG-FITC + platelet counter staining reagent-PE + permeabilization agent
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その他 |
[説明]VASP (Vasodilatator Stimulated Phosphoprotein) のリン酸化状態からP2Y12レセプターの反応性を測定する
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参考文献 |
"Comparison of VASP phosphorylation assay to light transmission aggregometry in assessing inhibition of the platelet ADP P2Y12 receptor" Pampuch et al.; Thromb Haemost. 2006 96:767-73 "Randomized comparison of a high clopidogrel maintenancedose in patients with diabetes mellitus and coronay artery disease" Angiolillo D. et al.; Circulation 2007. Cattaneo M.; "Platelet P2 receptors: old and new targets for antithrombotic drugs". Cardiovascular Therapy (2007) 5:45-55. Platelet Function Monitoring in Patients With Coronary Artery Disease; J Am Coll Cardiol 2007; 50:1822-34, Gurbel P et tal. Effect of caffeine on platelet inhibition by clopidogrel in healthy subjects and patients with coronary artery disease Am Heart J 2007, 154:4, 694 e1-7, Eli Lev et al, Relationships between optical aggregometry (type born) and flow cytometry in evaluating ADP-induced platelet activation. Sbrana S, Pina FD, Rizza A, Buffa M, De Filippis R, Gianetti J, Clerico A. Cytometry Part B 2008; 74B: 30-39. Influence of Omeprazole on the Antiplatelet Action of Clopidogrel Associated With Aspirin: The Randomized, Double-Blind OCLA (Omeprazole CLopidogrel Aspirin) Study Gilard M. et al., J. Am. Coll. Cardiol. 2008; 51: 256 - 260. Omeprazole A Possible New Candidate Influencing the Antiplatelet Effect of Clopidogrel Gurbel P.A. et al; JACC 2008; 51:261-3 A comparison of the antiplatelet effects of prasugrel and high-dose clopidogrel as assessed by VASP-phosphorylation andlight transmission aggregometry Jakubowski J. et al, Thromb Haemost. 2008;99:215-22 Relationship between aspirin and clopidogrel responses in acute coronary syndrome and clinical predictors of non response.Cuisset T et al, Thromb Res. 2008 , in press , Epub ahead of print P2Y12 Antagonism : Promises and Challenges, Michelson ATVB28,s33-38 Calcium-Channel Blockers Reduce the Antiplatelet Effect of Clopidogrel , Siller-Matula J.M.et al, J Am Coll Cardiol, 2008; 52:1557-1563 ADP-induced platelet aggregation frequently fails to detect impaired clopidogrel-responsiveness in patients with coronary artery disease compared to a P2Y12-specific assay. Sch臟er A et al, Thromb Haemost 2008; 100: 618-625 A randomized study assessing the impactof cilostazol on platelet function profiles in patients with diabetes mellitus and oronary artery disease on dual antiplatelet therapy: results of the OPTIMUS-2 study. Dominick J. Angiolillo et al. Eur Heart J. 2008 29:2202-11 Methods to Evaluate the Pharmacology of Oral Antiplatelet Drugs, Weber A.A. et al, Herz 2008;33:287-96. Thienopyridine Resistance Among Patients Undergoing Intracoronary Stent Implantation and Treated With Dual Antiplatelet Therapy: Assessment of Some Modifying Factors N. et al, Djukanovic N. et al, J Pharmacol Sci 107, 451 ? 455 (2008) Clinical implications of clopidogrel resistance De Miguel A. et al,Thromb Haemost 2008; 100: 196:203
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図1 VASPのリン酸化の模式図
図2 P2Y12(ADPレセプター)アンタゴニストのモニタリング方法
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メーカー |
品番 |
包装 |
BCT
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7014
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1 KIT [10 tests]
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※表示価格について
当社在庫 |
なし
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納期目安 |
2週間程度
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法規制 |
劇・安・化管
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保存温度 |
4℃
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