Anti VhH

Western Blot of Rabbit Anti VhH Antibody detecting VhH. Lane 1: MW Ladder Opal pre stained (p/n MB 210 0500). Lane 2: VhH. Load: 0.50 ug per lane. Primary Antibody: Anti VhH Antibody (p/n 200 401 GM6) at 1 ug/mL for overnight at 4C. Secondary Antibody: Rabbit Secondary HRP Antibody (p/n 611 103 122) at 1:70,000 for 30 min at RT. Block: MB 070 at RT for 30 min. Predicted/Observed size: ~15 kDa.

Western Blot of Rabbit anti-VHH antibody. Lane 1: Ladder (Opal Prestained). Lane 2: VHH protein (p/n 000-001-GM6). Lane 3: Llama IgG1 protein (p/n 025-0140). Lane 4: Llama IgG2 protein (p/n 025-0144). Lane 5: Llama IgG3 protein (p/n 025-0143). Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4C. Secondary antibody: Gt-a-Rb HRP (611-103-122) rabbit secondary antibody at 1:70,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.

Western Blot of Rabbit anti-VHH antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: Llama IgG1 protein (p/n 025-0140). Lane 3: Llama IgG2 protein (p/n 025-0144). Lane 4: Llama IgG3 protein (p/n 025-0143). Lane 5: VhH protein (p/n 000-001-GM6). Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:40,000 for 45 min at RT. Block: BlockOut (p/n MB-070) overnight at 4C. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.

Western Blot of Rabbit anti-VhH Antibody. Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 ug lysate with 25 ng VhH. Primary antibody: Rabbit anti-VhH Antibody at 1:1,000 o/n at 4C. Secondary antibody: HRP Gt-a-Rb IgG (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VhH.