| 別品名 |
single-domain antibody (sdAb), camelid VhH antibody
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| 精製度 |
IgG fraction
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Rabbit
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| 参考文献 |
Oliveira O, Rapid visualization of human tumor xenografts through optical imaging with a near-infrared fluorescent anti-epidermal growth factor receptor nanobody. Mol Imaging. 2012 Feb;11(1):33-46.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Results of Rabbit Anti VhH Antibody. Lane 1: Opal Prestained Molecular Weight (p/n MB 210 0500). Lane 2: Llama IgG1 isotype control (p/n 025 0140) reduced [0.05ug]. Lane 3: Llama IgG2 isotype control (p/n 025 0144) reduced [0.05ug]. Lane 4: Llama IgG3 isotype control (p/n 025 0143) reduced [0.05ug]. Lane 5: VhH Control Protein (p/n 000 001 GM6) reduced [0.05ug]. Primary Antibody: Anti VhH [Rabbit] Antibody at 1:1000 overnight at 2 8C. Secondary Antibody: Anti Rabbit IgG [Goat] Peroxidase Conjugated (p/n 611 1302) at 1:40,000 for 30mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB 070) for 1hr at RT. Expected: recognizes the VhH protein ~15kDa and detects the llama IgG1, IgG2 and IgG3 proteins.Exposure: 6.8 sec.
Western Blot of Rabbit anti-VhH Antibody. Lane 1: Hela whole cell lysate spiked with VhH single-domain antibody. Load: 10 ug lysate with 25 ng VhH. Primary antibody: Rabbit anti-VhH Antibody at 1:1,000 o/n at 4C. Secondary antibody: HRP Gt-a-Rb IgG (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VhH.
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Western Blot Results of Rabbit Anti VhH Antibody. Lane 1: Opal Prestained Molecular Weight (p/n MB 210 0500). Lane 2: Llama IgG1 isotype control (p/n 025 0140) reduced [0.05ug]. Lane 3: Llama IgG2 isotype control (p/n 025 0144) reduced [0.05ug]. Lane 4: Llama IgG3 isotype control (p/n 025 0143) reduced [0.05ug]. Lane 5: VhH Control Protein (p/n 000 001 GM6) reduced [0.05ug]. Primary Antibody: Anti VhH [Rabbit] Antibody at 1:1000 overnight at 2 8C. Secondary Antibody: Anti Rabbit IgG [Goat] Peroxidase Conjugated (p/n 611 1302) at 1:40,000 for 30mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n MB 070) for 1hr at RT. Expected: recognizes the VhH protein ~15kDa and detects the llama IgG1, IgG2 and IgG3 proteins.Exposure: 6.8 sec.
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| メーカー |
品番 |
包装 |
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RKL
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100-401-GM6
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100 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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