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※サムネイル画像をクリックすると拡大画像が表示されます。
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human synaptobrevin 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human VAMP1
Lane 2.: Recombinant Human VAMP2
Lane 3.: Recombinant Human VAMP3
The mouse brain (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human synaptobrevin 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse brain tissue lysate
The rat brain (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human synaptobrevin 2 antibody (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Rat brain tissue lysate
ICC/IF analysis of Synaptobrevin2 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Synaptobrevin2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
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The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human synaptobrevin 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human VAMP1
Lane 2.: Recombinant Human VAMP2
Lane 3.: Recombinant Human VAMP3
The mouse brain (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human synaptobrevin 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse brain tissue lysate
The rat brain (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human synaptobrevin 2 antibody (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Rat brain tissue lysate
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| 別品名 |
vesicle-associated membrane protein 2, VAMP2, SYB2, Synaptobrevin 2, VAMP-2, Synaptobrevin-2, Vesicle-associated membrane protein 2, vesicle-associated membrane protein 2 FLJ11460, RATVAMPB, RATVAMPIR, SYB, VAMP 2, Vesicle associated membrane protein 2, Vesicle-associated membrane protein 2 (synaptobrevin 2).
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| 種由来 |
Human
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| 交差種 |
Human
Mouse
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Immunocytochemistry (cell)
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| 免疫動物 |
Mouse
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| クローン |
3E5
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| 抗体クラス |
IgG1κ
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| 抗原部位 |
a.a.1-89
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein G
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| Accession No.(Gene/Protein) |
NP_055047, P63027
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| 概要 |
Synaptobrevin 2 (Vesicle-associated membrane, VAMP2), which is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal tail. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25. This antibody recognizes specifically synaptobrevin 2, but it also shows a low affinity to synaptobrevin 1.
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| 参考文献 |
Andres C et al.,(1995) Proc Natl Acad Sci USA 92:5987-5991
Jacobsson G et al.,(1998) European Journal of Neuroscience 16:301-316
Scales SJ. et al (2002) J Biol. Chem. 227(31):28271-28279
Pennuto M et al., (2003) Mol Biol Cell. 14:4909-19
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| メーカー |
品番 |
包装 |
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ATG
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AVA0710
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100 UL
[1mg/ml]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-７０℃
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