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※サムネイル画像をクリックすると拡大画像が表示されます。
The Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Brain lysate
Lane 2.: U87-MG cell lysate
ICC/IF analysis of PGP9.5 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGP9.5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Paraffin embedded sections of human nerve tissue were incubated with anti-human PGP9.5 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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The Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Brain lysate
Lane 2.: U87-MG cell lysate
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| 別品名 |
ubiquitin carboxyl-terminal esterase L1, uCHL1, PGP9.5, 1-223aa, uCH-L1, EC 3.4.19.12, ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, ubiquitin carboxyl-terminal hydrolase isozyme L1, ubiquitin carboxyl-terminal esterase L1 Gracile axonal dystrophy, Neuron cytoplasmic protein 9.5, Park 5, Parkinson Disease 5, PGP95, uCH L1, Protein gene product 9.5, ubiquitin C terminal esterase L1, ubiquitin C terminal hydrolase (neuron specific), ubiquitin carboxyl terminal esterase L1, ubiquitin carboxyl terminal hydrolase isozyme L1, ubiquitin thiolesterase, ubiquitin thiolesterase L1.
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
Immunocytochemistry (cell)
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| 免疫動物 |
Mouse
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| クローン |
3D9
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| 抗体クラス |
IgG2aκ
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| 抗原部位 |
a.a.1-223
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein G
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| Accession No.(Gene/Protein) |
NP_004172, P09936
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| 概要 |
PGP9. 5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9. 5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9. 5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.
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| 参考文献 |
kenji H et al , (1999) Am J Pathol 155(3):711-715
Liu Y et al , (2002) Cell 111(2):209-18
Mikako Sakurai.et al , (2006) Cell 119:162-171
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| メーカー |
品番 |
包装 |
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ATG
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APG0714
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100 UL
[1mg/ml]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-７０℃
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