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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: THP-1 cell lysate
Lane 3.: HeLa cell lysate
Lane 4.: U937 cell lysate
The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
ICC/IF analysis of MIF in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MIF antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of MIF in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MIF antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: THP-1 cell lysate
Lane 3.: HeLa cell lysate
Lane 4.: U937 cell lysate
The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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| 別品名 |
Macrophage migration inhibitory factor, GLIF, MMIF, MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor, macrophage migration inhibitory factor (glycosylation-inhibiting factor),
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Immunocytochemistry (cell)
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| 免疫動物 |
Mouse
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| クローン |
4E4
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| 抗体クラス |
IgG1κ
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| 抗原部位 |
a.a.1-114
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein G
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| Accession No.(Gene/Protein) |
NP_002406, P14174
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| 概要 |
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
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| 参考文献 |
Weiser WY, et al., (1989) Proc Natl Acad Sci. 86:7522-7526.
Bernhagen J, et al., (1994) Biochemistry. 33:14144-14155
Richard B, et al., (1996) FASEB J. 10:1607-1613
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| メーカー |
品番 |
包装 |
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ATG
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AMF0608
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100 UL
[1mg/ml]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-７０℃
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