Anti Pan Histone H3

Western blot analysis of Lane 1: recombinant Histone H3.3, Lane 2: recombinant Histone H3.1, Lane 3: A375, Lane 4: HEK293, Lane 5: HeLa and Lane 6: SK-MEL-2 whole cell lysate with Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) at 0.025 ug/mL working concentration, showed a band of recombinant Histone H3.3, recombinant Histone H3.1 and total Histone H3 in A375, HEK293, HeLa and SK-MEL-2 cells.

Immunocytochemical staining of HeLa cells with Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).

Sandwich ELISA analysis of HeLa whole cell lysate treated or untreated with Sodium Butyrate (NaBu) using Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804). A: Using Pan Histone H3 monoclonal antibody, clone RM188 as the capture antibody at 1 ug/mL working concentration and biotinylated format of Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) as the detection antibody at 1 ug/mL working concentration. B: Using Histone H3 (acetyl K79) monoclonal antibody, clone RM156 as the capture antibody at 5 ug/mL working concentration and biotinylated format of Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12837) as the detection antibody at 1 ug/mL working concentration.