UniGlow^TM - One Component Chemiluminescent Substrate  

UniGlow？ - One Component Chemiluminescent Substrate

Optimization results of the assay reagents. a？96 well plate assay results plotted on the right axis and Chemifluorescence assay result obtained from Optimizer？ microfluidic microplate is plotted on the left axis showing lower LOD and higher dynamic range and？b？Chemifluorescence signal output variation with increase in PfHRP2 capture antibody (CAb) concentration (bottom axis) and increase in PfHRP2 detection antibody (DAb-HRP) concentration (top axis) at a fixed DAb-HRP concentration (0.1？μg/mL) and at a fixed CAb concentration (100.0？μg/mL), respectively. Each point represents the mean of three replicates. Fig. 4. PMID: 34567620.

Cse4 ubiquitination is reduced in a？cse4 K65R？mutant. (A) Protein extracts were prepared from wild-type strain (BY4741) transformed with vector (pMB433),？pGAL-3HA-CSE4？(pMB1458), or？pGAL-3HA-cse4 K65R？(pMB1791) grown in raffinose/galactose (2%) for 4 hr to induce expression of Cse4. Agarose-TUBE1 was used to pull down tandem ubiquitin binding entities and ubiquitination levels of Cse4 were detected by western blot analysis with anti-HA antibody. Input samples were analyzed using anti-HA (Cse4) and anti-Tub2 antibodies. Asterisk shows non-modified Cse4.？One Component Chemiluminescent Substrate (p/n UniGlow-0100). Figure 2. PMID: ？29432128.