ManufacturerBioassayAlomone Labs PD 98059 inhibits P42/44 MAPK (ERK) phosphorylation via MEK in C6 glioma cells. - Cells were grown to 70% confluence and serum starved for 1.5 h. The cells were then incubated for 2 h with various concentrations of？PD 98059？(#P-260) and stimulated with 7 ng/ml PDGF-AA. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-P42/44 (upper panel) and with anti-P42/44 (lower panel) antibodies. Inhibition of P42/44 phosphorylation by PD 98059 was dose-dependent.
