種由来 |
Mouse
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発現系 |
Native
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純度 |
>95% (HPLC)
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分子量 |
26 kDa.
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CAS RN® |
93928-24-6
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化合物の概要 |
Native Mouse Nerve Growth Factor
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形状 |
凍結乾燥品
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その他 |
[内容・その他(内容)]mNGF 2.5S is purified from male mouse submaxillary glands by a modification of the procedure described by Darling and Shooter (1984)(1). It is a protein with a molecular weight of 26 kDa (a dimer of identical subunits).
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参考文献 |
1. Darling, T. and Shooter, E. M. (1984) "Methods for preparation and assay of nerve growth factor", Cell Culture Methods for Molecular and Cellular Biology, vol. 4 (Eds. D. W. Barnes, D.A. Sinbasku, and G.H. Sato), pp. 79-83, Alan R. Liss, New York. 2. Bradshaw, R. A. (1978) Ann. Rev. Biochem. 47, 191.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Alomone Labs Native mouse NGF?2.5S protein (>95%) promotes neurite outgrowth and survival?in PC12 cells. - Upper panel: PC12 cells were grown in absence of serum and in the presence of varying concentrations of Native?mouse NGF 2.5S protein (>95%)?(#N-100), the cell survival after 24 h was measured by XTT method and calculated as a relative percentage of the control and plotted against concentrations.?Lower panel:?PC12 cells were grown on collagen-coated plates in absence (1) or presence (2)?of 4?nM Native mouse NGF 2.5S protein (>95%).?The development of neurites over a period of?6 days was visualized by Methylene Blue staining.
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Alomone Labs Native mouse NGF?2.5S protein (>95%) promotes neurite outgrowth and survival?in PC12 cells. - Upper panel: PC12 cells were grown in absence of serum and in the presence of varying concentrations of Native?mouse NGF 2.5S protein (>95%)?(#N-100), the cell survival after 24 h was measured by XTT method and calculated as a relative percentage of the control and plotted against concentrations.?Lower panel:?PC12 cells were grown on collagen-coated plates in absence (1) or presence (2)?of 4?nM Native mouse NGF 2.5S protein (>95%).?The development of neurites over a period of?6 days was visualized by Methylene Blue staining.
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メーカー |
品番 |
包装 |
ALO
|
N-100
|
10 UG
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※表示価格について
当社在庫 |
なし
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入荷予定 |
あり
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納期目安 |
発注済・入荷予定
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保存温度 |
-20℃
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