| 別品名 |
CAF I p48 antibody|CAF-1 subunit C antibody|CAF-I 48 kDa subunit antibody|CAF-I p48 antibody|Chromatin assembly factor 1 subunit C antibody|Chromatin assembly factor I p48 subunit antibody|Chromatin assembly factor/CAF 1 p48 subunit antibody|Histone-binding protein RBBP4 antibody|MSI1 protein homolog antibody|Nucleosome-remodeling factor subunit RBAP48 antibody|NURF55 antibody|RbAp 48 antibody| RBAP48 antibody|RBBP-4 antibody|RBBP4 antibody|RBBP4_HUMAN antibody|Retinoblastoma binding protein 4 antibody|Retinoblastoma binding protein p48 antibody|Retinoblastoma-binding protein 4 antibody|Retinoblastoma-binding protein p48 antibody
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| 抗原部位 |
a.a.395-425, C-terminus
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
IHC paraffin embedding section
IHC frozen section
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 交差種 |
Human
Mouse
Rat
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| GENE ID |
5928
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| Accession No.(Gene/Protein) |
Q09028
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| Gene Symbol |
RBBP4
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| 分子量 |
47656 MW
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| 形状 |
凍結乾燥品
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| 参考文献 |
1. ''Entrez Gene: RBBP4 retinoblastoma-binding protein 4''.
2. Barak O, Lazzaro MA, Lane WS, Speicher DW, Picketts DJ, Shiekhattar R (November 2003). ''Isolation of human NURF: a regulator of Engrailed gene expression''. EMBO J 22 (22): 6089-100.
3. Qian YW, Wang YC, Hollingsworth RE Jr, Jones D, Ling N, Lee EY (September 1993). ''A retinoblastoma-binding protein related to a negative regulator of Ras in yeast''. Nature 364 (6438): 648-52.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot analysis of RbAp48 using anti-RbAp48 antibody (PB9797). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate,Lane 2: Mouse Liver Tissue Lysate,Lane 3: Mouse Lung Tissue Lysate,Lane 4: HELA Whole Cell Lysate,Lane 5: JURKAT Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RbAp48 antigen affinity purified polyclonal antibody (Catalog # PB9797) at 0.5 I？g/mL overnight at 4A°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RbAp48 at approximately 54KD. The expected band size for RbAp48 is at 54KD.
IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797).RbAp48 was detected in paraffin-embedded section of Mouse Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I？g/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797).RbAp48 was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I？g/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797).RbAp48 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I？g/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797).RbAp48 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I？g/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797).RbAp48 was detected in frozen section of mouse liver tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I？g/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797).RbAp48 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I？g/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797).RbAp48 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1I？g/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4A°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37A°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of RbAp48 using anti-RbAp48 antibody (PB9797). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate,Lane 2: Mouse Liver Tissue Lysate,Lane 3: Mouse Lung Tissue Lysate,Lane 4: HELA Whole Cell Lysate,Lane 5: JURKAT Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RbAp48 antigen affinity purified polyclonal antibody (Catalog # PB9797) at 0.5 I？g/mL overnight at 4A°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RbAp48 at approximately 54KD. The expected band size for RbAp48 is at 54KD.
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| メーカー |
品番 |
包装 |
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BBT
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PB9797
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 法規制 |
毒
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| 保存温度 |
-２０℃
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