Anti AGO2  

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA891731LA01HU at dilution of 1:100

IHC image of CSB-PA891731LA01HU diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA891731LA01HU diluted at 1:1200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western blot
All lanes: Protein argonaute-2 antibody at 2μg/ml
Lane 1: A549 whole cell lysate
Lane 2: Jurkats whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: Raw264.7 whole cell lysate
Lane 6: K562 whole cell lysate
Lane 7: Mouse liver tissue
Lane 8: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 98, 94 kDa
Observed band size: 94 kDa

Chromatin Immunoprecipitation Hela (1.1*10⁶) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-AgO2 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP891731HU) against the SLC1A5 promoter.