| 別品名 |
DKFZp686C01211 antibody; DNA binding factor KBF1 antibody; DNA binding factor KBF1 EBP1 antibody; DNA-binding factor KBF1 antibody; EBP 1 antibody; EBP-1 antibody; EBP1 antibody; KBF1 antibody; MGC54151 antibody; nf b antibody; NF kappa B antibody; NF kappaB antibody; NF kappabeta antibody; NF kB1 antibody; NF-kappaB antibody; NFkappaB antibody; NFKB 1 antibody; NFKB p105 antibody; NFKB p50 antibody; NFKB-p105 antibody; Nfkb1 antibody; NFKB1_HUMAN antibody; Nuclear factor kappa B DNA binding subunit antibody; Nuclear factor kappa-B antibody; Nuclear factor kappa-B, subunit 1 antibody; Nuclear factor NF kappa B p105 subunit antibody; Nuclear factor NF kappa B p50 subunit antibody; Nuclear factor NF-kappa-B p50 subunit antibody; Nuclear factor of kappa light chain gene enhancer in B cells 1 antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody; p105 antibody; p50 antibody; p84/NF-kappa-B1 p98 antibod
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| 抗原部位 |
a.a.1-961
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein G
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
Chromatin Immunoprecipitation
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 交差種 |
Human
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| Accession No.(Gene/Protein) |
P19838
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| 形状 |
液状
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| 参考文献 |
[1] "The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product."Kieran M., Blank V., Logeat F., Vandekerckhove J., Lottspeich F., le Bail O., Urban M.B., Kourilsky P., Baeuerle P.A., Israel A.Cell 62:1007-1018(1990) [PubMed: 2203531] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PARTIAL PROTEIN SEQUENCE. [2] "Cloning of a mitogen-inducible gene encoding a kappa B DNA-binding protein with homology to the rel oncogene and to cell-cycle motifs."Bours V., Villalobos J., Burd P.R., Kelly K., Siebenlist U.Nature 348:76-80(1990) [PubMed: 2234062] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. [3] "Cloning of the DNA-binding subunit of human nuclear factor kappa B: the level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor alpha."Meyer R., Hatada E.N., Hohmann H.-P., Haiker M., Bartsch C., Roethlisberger U., Lahm H.-W., Schlaeger E.J., van Loon A.P.G.M., Scheidereit C.Proc. Natl. Acad. Sci. U.S.A. 88:966-970(1991) [PubMed: 1992489] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM1), PARTIAL PROTEIN SEQUENCE.
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Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: NFKB1 antibody at 3.75μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 106, 86 kDa
Observed band size: 106 kDa
IHC image of CSB-PA10354A0Rb diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA10354A0Rb diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with CSB-PA10354A0Rb at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-NFKB1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP10354HU) against the IkB promoter.
IHC image of CSB-PA10354A0Rb diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.
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Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: NFKB1 antibody at 3.75μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 106, 86 kDa
Observed band size: 106 kDa
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| メーカー |
品番 |
包装 |
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CSB
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CSB-PA10354A0RB
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50 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
2週間程度
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| 保存温度 |
-２０℃
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