別品名 |
HD3 antibody; HDAC 3 antibody; HDAC3 antibody; HDAC3_HUMAN antibody; Histone deacetylase 3 antibody; RPD3 2 antibody; RPD3 antibody; RPD3-2 antibody; SMAP45 antibody
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抗原部位 |
a.a.1-428
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Ig fraction - Protein G
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry Immuno Fluorescence Chromatin Immunoprecipitation
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse
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Accession No.(Gene/Protein) |
O15379
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形状 |
液状
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参考文献 |
[1]"A hybrid mechanism of action for BCL6 in B cells defined by formation of functionally distinct complexes at enhancers and promoters." Hatzi K., Jiang Y., Huang C., Garrett-Bakelman F., Gearhart M.D., Giannopoulou E.G., Zumbo P., Kirouac K., Bhaskara S., Polo J.M., Kormaksson M., Mackerell A.D. Jr., Xue F., Mason C.E., Hiebert S.W., Prive G.G., Cerchietti L., Bardwell V.J., Elemento O., Melnick A. Cell Rep. 4:578-588(2013) . [2]"Acetylation of a conserved lysine residue in the ATP binding pocket of p38 augments its kinase activity during hypertrophy of cardiomyocytes." Pillai V.B., Sundaresan N.R., Samant S.A., Wolfgeher D., Trivedi C.M., Gupta M.P. Mol. Cell. Biol. 31:2349-2363(2011). [3]"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011).
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Western blot All lanes: HDAC3 antibody at 2μg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 50 kDa Observed band size: 49 kDa
Western Blot Positive WB detected in: Mouse heart tissue All lanes: HDAC3 antibody at 2.5μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 50 kDa Observed band size: 49 kDa
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA010239LA01HU at dilution of 1:100
Chromatin Immunoprecipitation Hela(1.2*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-HDAC3 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP010239HU) against the P21 promoter.
Immunofluorescent analysis of Hela cells using CSB-PA010239LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western blot All lanes: HDAC3 antibody at 2μg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 50 kDa Observed band size: 49 kDa
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メーカー |
品番 |
包装 |
CSB
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CSB-PA010239LA01HU
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50 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
2週間程度
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保存温度 |
-20℃
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