別品名 |
Apoptosis regulator Bcl X antibody; Apoptosis regulator Bcl-X antibody; Apoptosis regulator BclX antibody; B cell lymphoma 2 like antibody; B2CL1_HUMAN antibody; Bcl 2 like 1 protein antibody; Bcl X antibody; Bcl xL antibody; BCL XL/S antibody; Bcl xS antibody; Bcl-2-like protein 1 antibody; Bcl2 Like 1 antibody; Bcl2 related gene antibody; Bcl2-L-1 antibody; BCL2L antibody; Bcl2l1 antibody; BCLX antibody; BclXL antibody; BclXs antibody; DKFZp781P2092 antibody; PPP1R52 antibody; Protein phosphatase 1 regulatory subunit 52 antibody
|
抗原部位 |
a.a.1-209
|
種由来 |
Human
|
標識物 |
Unlabeled
|
精製度 |
Ig fraction - Protein G
|
適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry Immuno Fluorescence
|
免疫動物 |
Rabbit
|
抗体クラス |
IgG
|
交差種 |
Human Mouse
|
Accession No.(Gene/Protein) |
Q07817
|
形状 |
液状
|
参考文献 |
[1]PUMA binding induces partial unfolding within BCL-xL to disrupt p53 binding and promote apoptosis. Follis A.V., Chipuk J.E., Fisher J.C., Yun M.K., Grace C.R., Nourse A., Baran K., Ou L., Min L., White S.W., Green D.R., Kriwacki R.W. Nat. Chem. Biol. 9:163-168(2013). [2]Structural changes in the BH3 domain of SOUL protein upon interaction with the anti-apoptotic protein Bcl-xL. Ambrosi E., Capaldi S., Bovi M., Saccomani G., Perduca M., Monaco H.L. Biochem. J. 438:291-301(2011). [3]Studies leading to potent, dual inhibitors of Bcl-2 and Bcl-xL. Bruncko M., Oost T.K., Belli B.A., Ding H., Joseph M.K., Kunzer A., Martineau D., McClellan W.J., Mitten M., Ng S.-C., Nimmer P.M., Oltersdorf T., Park C.-M., Petros A.M., Shoemaker A.R., Song X., Wang X., Wendt M.D. expand/collapse author list Elmore S.W. J. Med. Chem. 50:641-662(2007).
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot Positive WB detected in: RAW264.7 whole cell lysate All lanes: BCL2L1 antibody at 2.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 27, 19, 26 kDa Observed band size: 27 kDa
IHC image of CSB-PA002613HA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA002613HA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with CSB-PA002613HA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
|
|
Western Blot Positive WB detected in: RAW264.7 whole cell lysate All lanes: BCL2L1 antibody at 2.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 27, 19, 26 kDa Observed band size: 27 kDa
|
|
|
メーカー |
品番 |
包装 |
CSB
|
CSB-PA002613HA01HU
|
50 UG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
2週間程度
|
保存温度 |
-20℃
|
|