Anti p53, phospho Ser315  

Fig.1 Identification of Ser315-phosphorylated p53 proteinby Western blotting.
Sample: Crude cell extracts of MCF7 untreated (lane 1)　and treated with nocodazole at 100 ng/ml for 48 h (lane2).
The lower panel is the whole p53 protein identified by　omnipotent anti-p53 antibody (DO-1).

Fig.2 Immunohistochemistry of stomach cancer.
(Formalin/PFA-fixed paraffin-embedded section)

Fig.3 Immunofluorescence staining of p53 phosho-Ser315 in nuclei of HeLa cells subjected DNA damage.
1. HeLa cells were treated with 100 nM Doxorubicin for 24 hr, fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min.
2. Incubate cells with 1.5% BSA in PBS for 30 min to block unspecific binding of the antibodies. Incubate the cells with 1/1,000 diluted anti-p53(p-315) antibody in 1% BSA in PBS at 4℃ overnight.
3. Incubate cells with the secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature.
4. Nucleus (DNA) was stained with DAPI