| 別品名 |
Keratin 5, CK5, DDD, K5, KRT5A
|
| 抗原部位 |
a.a.1-590
|
| 種由来 |
Human
|
| 標識物 |
Unlabeled
|
| 精製度 |
Ig fraction - Protein A
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
Immunocytochemistry (cell)
Flow Cytometry
|
| 免疫動物 |
Mouse
|
| 抗体クラス |
IgG2aκ
|
| クローン |
AT12C4
|
| 交差種 |
Human
|
| Accession No.(Gene/Protein) |
NP_000415, P13647
|
| 形状 |
液状
|
| 参考文献 |
Lersch R., et al. (1988) Molecular and Cellular Biology. 8(1): 486-493.
Eckert RL., et al. (1988) Dna. 7(5): 337-345.
Atkinson SD., et al. (2011) J Invest Dermatol. 131(10): 2079-2086.
van de Rijn M., et al. (2002) Am J Pathol. 161(6): 1991-1996.
|
|
※サムネイル画像をクリックすると拡大画像が表示されます。
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : A431 cell lysate
ICC/IF analysis of KRT5 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Paraffin embedded sections of human cervical cancer tissue were incubated with anti-human KRT5 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
|
|
|
|
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : A431 cell lysate
|
|
|
| メーカー |
品番 |
包装 |
|
ATG
|
ATGA0368
|
100 UL
[1mg/ml]
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
1週間程度
|
| 保存温度 |
-７０℃
|
|
