|
※サムネイル画像をクリックすると拡大画像が表示されます。
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : A431 cell lysate
ICC/IF analysis of KRT5 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Paraffin embedded sections of human cervical cancer tissue were incubated with anti-human KRT5 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
|
|
|
|
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : A431 cell lysate
|
|
| 別品名 |
Keratin 5, CK5, DDD, K5, KRT5A
|
| 種由来 |
Human
|
| 交差種 |
Human
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
Immunocytochemistry (cell)
Flow Cytometry
|
| 免疫動物 |
Mouse
|
| クローン |
AT12C4
|
| 抗体クラス |
IgG2aκ
|
| 抗原部位 |
a.a.1-590
|
| 標識物 |
Unlabeled
|
| 精製度 |
Ig fraction - Protein A
|
| Accession No.(Gene/Protein) |
NP_000415, P13647
|
| 概要 |
Keratin 5, also known as KRT5, K5, or CK5, dimerizes with keratin 14 and forms the intermediate filaments (IF) that make up the cytoskeleton of basal epithelial cells. This protein is involved in several diseases including epidermolysis bullosa simplex and breast and lung cancers. Keratin 5 (and K14) are expressed primarily in basal keratinocytes in the epidermis, specifically in the stratified epithelium lining the skin and digestive tract. Keratin intermediate filaments make up the cytoskeletal scaffold within epithelial cells, which contributes to the cell architecture and provides the cells with the ability to withstand mechanical, and non-mechanical, stresses. K5/K14 keratin pairs are able to undergo extensive bundling due to the non-helical tail of K15 acting as a weak cross-linker at the intermediate filament surface. This bundling increase the elasticity, and therefore the mechanical resilience, of the intermediate filaments.
|
| 参考文献 |
Lersch R., et al. (1988) Molecular and Cellular Biology. 8(1): 486-493.
Eckert RL., et al. (1988) Dna. 7(5): 337-345.
Atkinson SD., et al. (2011) J Invest Dermatol. 131(10): 2079-2086.
van de Rijn M., et al. (2002) Am J Pathol. 161(6): 1991-1996.
|
|
| メーカー |
品番 |
包装 |
|
ATG
|
ATGA0368
|
50 UL
[1mg/ml]
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
1週間程度
|
| 保存温度 |
-７０℃
|
|
※当社では商品情報の適切な管理に努めておりますが、表示される法規制情報は最新でない可能性があります。
また法規制情報の表示が無いものは、必ずしも法規制に非該当であることを示すものではありません。
商品のお届け前に最新の製品法規制情報をお求めの際はこちらへお問い合わせください。
|
※当社取り扱いの試薬・機器製品および受託サービス・創薬支援サービス（納品物、解析データ等）は、研究用としてのみ販売しております。
人や動物の医療用・臨床診断用・食品用としては、使用しないように、十分ご注意ください。
法規制欄に体外診断用医薬品と記載のものは除きます。
|
|
※リンク先での文献等のダウンロードに際しましては、掲載元の規約遵守をお願いします。
|
