別品名 |
Keratin 5, CK5, DDD, K5, KRT5A
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抗原部位 |
a.a.1-590
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Ig fraction - Protein A
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry Immuno Fluorescence Immunocytochemistry (cell) Flow Cytometry
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免疫動物 |
Mouse
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抗体クラス |
IgG2aκ
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クローン |
AT12C4
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交差種 |
Human
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Accession No.(Gene/Protein) |
NP_000415, P13647
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形状 |
液状
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参考文献 |
Lersch R., et al. (1988) Molecular and Cellular Biology. 8(1): 486-493. Eckert RL., et al. (1988) Dna. 7(5): 337-345. Atkinson SD., et al. (2011) J Invest Dermatol. 131(10): 2079-2086. van de Rijn M., et al. (2002) Am J Pathol. 161(6): 1991-1996.
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※サムネイル画像をクリックすると拡大画像が表示されます。
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate Lane 2. : A431 cell lysate
ICC/IF analysis of KRT5 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Paraffin embedded sections of human cervical cancer tissue were incubated with anti-human KRT5 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate Lane 2. : A431 cell lysate
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メーカー |
品番 |
包装 |
ATG
|
ATGA0368
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50 UL [1mg/ml]
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※表示価格について
当社在庫 |
なし
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納期目安 |
1週間程度
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保存温度 |
-70℃
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