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※サムネイル画像をクリックすると拡大画像が表示されます。
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD14 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: A549 cell lysate
Flow cytometry
Flow cytometry analysis of PBMC, staining CD14. The sample was incubated with the primary antibody at 1:100 for 30min, 4C. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Gating Strategy: Lymphocyte
Flow cytometry
Flow cytometry analysis of CD14 in Human THP-1 cell line, staining at 1:100 dilution (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD14 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: A549 cell lysate
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| 別品名 |
monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
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| 種由来 |
Human
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Flow Cytometry
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| 免疫動物 |
Mouse
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| クローン |
AT87H7
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| 抗体クラス |
IgG2bκ
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| 抗原部位 |
a.a.20-349
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| Accession No.(Gene/Protein) |
NP_001167576, P08571
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| 概要 |
The protein encoded by this gene is a component of the innate immune system. CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol tail (mCD14), the other a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48kDa) or is directly secreted from intracellular vesicles (56kDa). The x-ray crystal structure of human CD14 reveals a monomeric, bent solenoid structure containing a hydrophobic amino-terminal pocket. CD14 was the first described pattern recognition receptor. CD14 acts as a co-receptor (along with the Toll-like receptor TLR4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns such as lipoteichoic acid.
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| 参考文献 |
Kirkland T.N., et al. (1998) Prog Clin Biol Res. 397: 79-87
Kelley S.L., et al. (2013) Journal of Immunology. 190(3): 1304-1311.
Kitchens R.L., et al. (2000) Chem Immunol Chemical Immunology and Allergy. 74: 61-82
Tapping R.I., et al. (2000) Chem Immunol Chemical Immunology and Allergy. 74: 108-121
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| メーカー |
品番 |
包装 |
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ATG
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ATGA0339
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100 UL
[1mg/ml]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-７０℃
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