| 構成内容 |
A. ReadiView Biotin Succinimidyl Ester (Cat. # 3059) 3 vials Store at 4 °C; avoid moisture
B. Reaction Buffer (pH 8.0) 3x0.5 mL Do not freeze
C. Pre-packed Purification Column 3 columns Do not freeze
D. DMSO for Preparing Biotin Solution 3 mL Avoid moisture
E. 50X Elution Buffer 10 mL Do not freeze
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| 参考文献 |
Gabriel L, Stevens Z, Melikian H.. (2009). Measuring plasma membrane protein endocytic rates by reversible biotinylation. J Vis Exp., .
Garanger E, Weissleder R, Josephson L.. (2009). A multifunctional single-attachment-point reagent for controlled protein biotinylation. Bioconjug Chem, 20, 170.
Kim J, Cantor AB, Orkin SH, Wang J.. (2009). Use of in vivo biotinylation to study protein-protein and protein-DNA interactions in mouse embryonic stem cells. Nat Protoc, 4, 506.
Volkmann G, Liu XQ.. (2009). Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein. PLoS One, 4, e8381.
Wang J, Cantor AB, Orkin SH.. (2009). Tandem affinity purification of protein complexes in mouse embryonic stem cells using in vivo biotinylation. Curr Protoc Stem Cell Biol, Chapter 1, Unit1B 5.
Bagautdinov B, Matsuura Y, Bagautdinova S, Kunishima N.. (2008). Protein biotinylation visualized by a complex structure of biotin protein ligase with a substrate. J Biol Chem, 283, 14739.
Conrotto P, Roesli C, Rybak J, Kischel P, Waltregny D, Neri D, Castronovo V.. (2008). Identification of new accessible tumor antigens in human colon cancer by ex vivo protein biotinylation and comparative mass spectrometry analysis. Int J Cancer, 123, 2856.
Fernandez-Suarez M, Chen TS, Ting AY.. (2008). Protein-protein interaction detection in vitro and in cells by proximity biotinylation. J Am Chem Soc, 130, 9251.
Kim WS, Elliott DA, Kockx M, Kritharides L, Rye KA, Jans DA, Garner B.. (2008). Analysis of apolipoprotein E nuclear localization using green fluorescent protein and biotinylation approaches. Biochem J, 409, 701.
Link AJ, Labaer J.. (2008). Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 4: DNA Biotinylation, Precipitation, and Arraying of Samples. CSH Protoc, 2008, pdb prot5059.
Shi XW, Liu Y, Lewandowski AT, Wu LQ, Wu HC, Ghodssi R, Rubloff GW, Bentley WE, Payne GF.. (2008). Chitosan biotinylation and electrodeposition for selective protein assembly. Macromol Biosci, 8, 451.
Viens A, Harper F, Pichard E, Comisso M, Pierron G, Ogryzko V.. (2008). Use of protein biotinylation in vivo for immunoelectron microscopic localization of a specific protein isoform. J Histochem Cytochem, 56, 911.
Vila A, Tallman KA, Jacobs AT, Liebler DC, Porter NA, Marnett LJ.. (2008). Identification of protein targets of 4-hydroxynonenal using click chemistry for ex vivo biotinylation of azido and alkynyl derivatives. Chem Res Toxicol, 21, 432.
Winkler DF, McGeer PL.. (2008). Protein labeling and biotinylation of peptides during spot synthesis using biotin p-nitrophenyl ester (biotin-ONp). Proteomics, 8, 961.
Basile G, Peticca M, Catello S.. (2007). Site-specific biotinylation of human myeloid differentiation protein 88 in Drosophila melanogaster Schneider 2 cell cytoplasm. Mol Biotechnol, 35, 253.
Blazer LL, Boyle MD.. (2007). Use of protein chip mass spectrometry to monitor biotinylation reactions. Appl Microbiol Biotechnol, 74, 717.
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| 使用文献 |
Gabriel L Stevens Z, Melikian H. (2009). Measuring plasma membrane protein endocytic rates by reversible biotinylation. J Vis Exp.
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| メーカー |
品番 |
包装 |
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ABD
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5521
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1 KIT
[3 Reactions]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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