励起波長 |
350
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蛍光波長 |
450
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検出系 |
蛍光
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使用目的 |
F-Actin標識用キットです。青色蛍光色素ファロイジンがF-Actinに選択的に結合します。DAPIフィルターで蛍光観察が可能で、ホルムアルデヒド固定・透過処理された組織切片、細胞または無細胞の実験系でお使いいただけます。
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構成内容 |
・Component A, iFluorTM 350-Phalloidin, 1 vial ・Component B, Labeling Buffer, 50 mL ・Component C, Methanol, 1 vial (1.5 mL)
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参考文献 |
Haghparast, Seyed Mohammad Ali and Kihara, Takanori and Shimizu, Yuji and Yuba, Shunsuke and Miyake, Jun. (2013). Actin-based biomechanical features of suspended normal and cancer cells. Journal of bioscience and bioengineering, 116(3), 380--385. Haghparast, Seyed Mohammad Ali and Kihara, Takanori and Miyake, Jun. (2015). Distinct mechanical behavior of HEK293 cells in adherent and suspended states. PeerJ, 3, e1131. Pan, Ting and Song, Wenjing and Gao, Huichang and Li, Tianjie and Cao, Xiaodong and Zhong, Shizhen and Wang, Yingjun. (2016). miR-29b-Loaded Gold Nanoparticles Targeting to the Endoplasmic Reticulum for Synergistic Promotion of Osteogenic Differentiation. ACS Applied Materials & Interfaces, 8(30), 19217--19227. Song, Juqing and Gao, Huichang and Zhu, Guanglin and Cao, Xiaodong and Shi, Xuetao and Wang, Yingjun. (2015). The preparation and characterization of polycaprolactone/graphene oxide biocomposite nanofiber scaffolds and their application for directing cell behaviors. Carbon, 95, 1039--1050. Yang, Huishang and Zhu, Guanglin and Huang, Yicheng and Shi, Xuetao and Wang, Yingjun. (2016). The stimulation of the differentiation of pheochromocytoma (PC12-L) cells into neuron-like cells by electrically conductive nanofibre mesh. Applied Materials Today, 5, 215--222. Lin, Zifeng and Zhao, Xiujuan and Chen, Si and Du, Chang. (2016). Osteogenic and tenogenic induction of hBMSCs by integrated nanofibrous scaffold with chemical and structural mimic to bone-ligament connection. Journal of Materials Chemistry B, . Li, Xingmei and He, Fupo and Ye, Jiandong. (2017). Preparation, characterization and in vitro cell performance of anti-washout calcium phosphate cement modified by sodium polyacrylate. RSC Advances, 7(52), 32842--32849. Guo, Xiaoheng and Gao, Huichang and Liu, Xiao and Diao, Jingjing and Shi, Xuetao and Zhao, Naru and Wang, Yingjun. (2017). Porous Li-containing biphasic calcium phosphate scaffolds fabricated by three-dimensional plotting for bone repair. RSC Advances, 7(55), 34508--34516.
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使用文献 |
Wan S, Fu X, Ji Y, Li M, Shi X, Wang Y. (2018). FAK-and YAP/TAZ dependent mechanotransduction pathways are required for enhanced immunomodulatory properties of adipose-derived mesenchymal stem cells induced by aligned fibrous scaffolds. Biomaterials, 107--117.
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[注意事項] |
【安衛法】この商品は、労働安全衛生法で定める皮膚等障害化学物質等を含みます。
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※サムネイル画像をクリックすると拡大画像が表示されます。
図1. 本キット(品番22660)を用いて染色したCPA細胞(ホルマリン固定) (A) iFluor? 350-Phalloidin で30 分間染色した。 (B) phalloidinで10分間細胞を処理した後、iFluor? 350-Phalloidinで30分間染色した。
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図1. 本キット(品番22660)を用いて染色したCPA細胞(ホルマリン固定) (A) iFluor? 350-Phalloidin で30 分間染色した。 (B) phalloidinで10分間細胞を処理した後、iFluor? 350-Phalloidinで30分間染色した。
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メーカー |
品番 |
包装 |
ABD
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22660
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1 KIT [500 Tests]
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※表示価格について
当社在庫 |
なし
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納期目安 |
1週間程度
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法規制 |
劇・安
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保存温度 |
-20℃
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