| 検出系 |
蛍光
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| 溶媒 |
DMSO
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| 使用目的 |
スライドイメージング用の光退色防止剤です。本キットには、イメージングのタイプよって使い分けていただける、3種類のready-to-useの試薬が含まれています。
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| 構成内容 |
・Component A Optimized for FITC and other fluorescein-based imaging experiments 1ml.
・Component B Optimized for multiplexing imaging. In some cases, it enhances initial fluorescence intensity besides its anti-fading effect 1ml.
・Component C Optimized for multiplexing imaging with minimal phototoxicity 1 mL.
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| 参考文献 |
Benchaib M, Delorme R, Pluvinage M, Bryon PA, Souchier C.. (1996). Evaluation of five green fluorescence-emitting streptavidin-conjugated fluorochromes for use in immunofluorescence microscopy. Histochem Cell Biol, 106, 253.
Cordes T, Vogelsang J, Tinnefeld P.. (2009). On the mechanism of Trolox as antiblinking and antibleaching reagent. J Am Chem Soc, 131, 5018.
Gallardo-Escarate C, Alvarez-Borrego J, Von Brand E, Dupre E, Del Rio-Portilla MA.. (2007). Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification. Biol Res, 40, 29.
Widengren J, Chmyrov A, Eggeling C, Lofdahl PA, Seidel CA.. (2007). Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy. J Phys Chem A, 111, 429.
Dikicioglu E, Meteoglu I, Okyay P, Culhaci N, Kacar F.. (2003). The reliability of long-term storage of direct immunofluorescent staining slides at room temperature. J Cutan Pathol, 30, 430.
El-Rifai WE, Knuutila S.. (2001). Comparative genomic hybridization technique. Methods Mol Med, 50, 25.
Berrios M, Conlon KA, Colflesh DE.. (1999). Antifading agents for confocal fluorescence microscopy. Methods Enzymol, 307, 55.
Brock R, Hamelers IH, Jovin TM.. (1999). Comparison of fixation protocols for adherent cultured cells applied to a GFP fusion protein of the epidermal growth factor receptor. Cytometry, 35, 353.
Kolanko CJ, Pyle MD, Loats H, Parton J, Blakely WF, Nath J.. (1999). Fast-in situ hybridization and immunoenzymatic color pigment detection of mouse bone marrow micronucleus. Biotech Histochem, 74, 111.
Swartz DJ, Santi PA.. (1996). Immunofluorescent artifacts due to the pH of antifading mounting media. Biotechniques, 20, 398.
Abend M, Blakely WF, van Beuningen D.. (1995). Simplified and optimized kinetochore detection: cytogenetic marker for late-G2 cells. Mutat Res, 334, 39.
Florijn RJ, Slats J, Tanke HJ, Raap AK.. (1995). Analysis of antifading reagents for fluorescence microscopy. Cytometry, 19, 177.
Horiuchi K, Osterhout ML, Kamma H, Bekoe NA, Hirokawa KJ.. (1995). Estimation of fetal hemoglobin levels in individual red cells via fluorescence image cytometry. Cytometry, 20, 261.
Russell SD.. (1990). A method for improved resolution for fluorescence microscopy using plastic-embedded material subjected to resin extraction. Stain Technol, 65, 259.
Krenik KD, Kephart GM, Offord KP, Dunnette SL, Gleich GJ.. (1989). Comparison of antifading agents used in immunofluorescence. J Immunol Methods, 117, 91.
Bock G, Hilchenbach M, Schauenstein K, Wick G.. (1985). Photometric analysis of antifading reagents for immunofluorescence with laser and conventional illumination sources. J Histochem Cytochem, 33, 699.
Oriol R, Mancilla-Jimenez R.. (1983). Fluorescent staining of nuclei and amyloid substance. Two useful properties of p-phenylenediamine. J Immunol Methods, 62, 185.
Spatz WB, Grabig S.. (1983). Reduced fading of Fast Blue fluorescence in the brain of the guinea-pig by treatment with sodium-nitroprusside. Neurosci Lett, 38, 1.
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| 使用文献 |
Cordes T Vogelsang J, Tinnefeld P. (2009). On the mechanism of Trolox as antiblinking and antibleaching reagent. J Am Chem Soc, 5018.
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| メーカー |
品番 |
包装 |
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ABD
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20001
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1 KIT
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 保存温度 |
-２０℃
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