| 励起波長 |
540
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| 蛍光波長 |
590
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| 検出系 |
蛍光
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| 使用目的 |
本キットにはMMPの基質として TF2TM/TQ2TM FRET を利用し、MMP活性の測定を行います。MMP活性により TF2TM/TQ2TM FRET ペプチドは分断され、それまで TQ2TM によって消光されていた TF2TMが蛍光します。EDANS/Dabcyl FRETよりも高感度です。
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| 構成内容 |
・MMP ReTM基質 1 vial (60 μL), protected from light
・APMA, 4-Aminophenylmercuric Acetate 1 vial (20 μL, 1 M)
・アッセイバッファー 1 bottle (20 mL)
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| 参考文献 |
Taddei, Maria Letizia and Parri, Matteo and Angelucci, Adriano and Bianchini, Francesca and Marconi, Chiara and Giannoni, Elisa and Raugei, Giovanni and Bologna, Mauro and Calorini, Lido and Chiarugi, Paola. (2011). EphA2 induces metastatic growth regulating amoeboid motility and clonogenic potential in prostate carcinoma cells. Molecular Cancer Research, 9(2), 149--160.
Przybyla, Laralynne M and Theunissen, Thorold W and Jaenisch, Rudolf and Voldman, Joel. (2013). Matrix Remodeling Maintains Embryonic Stem Cell Self-Renewal by Activating Stat3. Stem Cells, 31(6), 1097--1106.
Delic, S and Lottmann, N and Jetschke, K and Reifenberger, G and Riemenschneider, Markus J. (2012). Identification and functional validation of CDH11, PCSK6 and SH3GL3 as novel glioma invasion-associated candidate genes. Neuropathology and applied neurobiology, 38(2), 201--212.
Duan, Qiong and Mao, Xiaoxiao and Liao, Chaonan and Zhou, Haoyang and Sun, Zelin and Deng, Xu and Hu, Qiuning and Qi, Jun and Zhang, Guogang and Huang, He and others. (2016). Inhibition of BET bromodomain attenuates angiotensin II induced abdominal aortic aneurysm in ApoE-/- mice. International Journal of Cardiology, 223, 428--432.
Mandel, Erin R and Uchida, Cassandra and Nwadozi, Emmanuel and Makki, Armin and Haas, Tara L. (2016). Tissue inhibitor of metalloproteinase 1 influences vascular adaptations to chronic alterations in blood flow. Journal of Cellular Physiology, .
Moretti, Silvia and Massi, Daniela and Farini, Valentina and Baroni, Gianna and Parri, Matteo and Innocenti, Stefania and Cecchi, Roberto and Chiarugi, Paola. (2013). Β-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines. Laboratory Investigation, 93(3), 279--290.
Kou, Yu and Ji, Liyan and Wang, Haojia and Wang, Wensheng and Zheng, Hongming and Zou, Juan and Liu, Linxin and Qi, Xiaoxiao and Liu, Zhongqiu and Du, Biaoyan and others. (2017). Connexin 43 Upregulation by Dioscin Inhibits Melanoma Progression via Suppressing Malignancy and Inducing M1 Polarization. International Journal of Cancer, .
Kittur, Harsha and Tay, Andy and Hua, Avery and Yu, Min and Di Carlo, Dino. (2017). Probing Cell Adhesion Profiles with a Microscale Adhesive Choice Assay. Biophysical Journal, 113(8), 1858--1867.
Lu, Linlin and Wang, Ying and Ou, Rilan and Feng, Qian and Ji, Liyan and Zheng, Hongming and Guo, Yue and Qi, Xiaoxiao and Kong, Ah-Ng Tony and Liu, Zhongqiu. (2017). DACT2, an epigenetic stimulator, exerts dual efficacy for colorectal cancer prevention and treatment. Pharmacological research, .
Xiao, Ruyue and Yuan, Lan and He, Weijiang and Yang, Xiaoda. (2018). Zinc ions regulate opening of tight junction favouring efflux of macromolecules via the GSK3β/snail-mediated pathway. Metallomics, .
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| 使用文献 |
Xiao Ruyue, Yuan Lan, He Weijiang, Yang Xiaoda. (2018). Zinc ions regulate opening of tight junction favouring efflux of macromolecules via the GSK3β/snail-mediated pathway. Metallomics.
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Amplite Universal Fluorimetric MMP Activity Assay Kit によるMMPs活性測定
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Amplite Universal Fluorimetric MMP Activity Assay Kit によるMMPs活性測定
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| メーカー |
品番 |
包装 |
|
ABD
|
13511
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1 KIT
[100 Tests]
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
1週間程度
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| 法規制 |
毒・安・化管
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| 保存温度 |
-２０℃
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