励起波長 |
540
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蛍光波長 |
540
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検出系 |
発色/蛍光
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使用目的 |
蛍光のHRP基質(Amplite Red peroxidase substrate)により溶液中のHRP活性を定量します。
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構成内容 |
・AmpliteTM Red Peroxidase基質 1 vial ・H2O2 1 vial (3% stabilized solution, 200 μL) ・アッセイバッファー 1 bottle (100 mL) ・Horseradish Peroxidase 1 vial (20 units) ・DMSO 1 vial (1 mL)
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参考文献 |
Almeida LE, Imasato H, Tabak M.. (2006). Enzymatic oxidation of dipyridamole in homogeneous and micellar solutions in the horseradish peroxidase-hydrogen peroxide system. Biochim Biophys Acta, 1760, 216. Didenko VV, Baskin DS.. (2006). Horseradish peroxidase-driven fluorescent labeling of nanotubes with quantum dots. Biotechniques, 40, 295. Krieg R, Halbhuber KJ.. (2003). Recent advances in catalytic peroxidase histochemistry. Cell Mol Biol (Noisy-le-grand), 49, 547. Matsui T, Nakayama H, Yoshida K, Shinmyo A.. (2003). Vesicular transport route of horseradish C1a peroxidase is regulated by N- and C-terminal propeptides in tobacco cells. Appl Microbiol Biotechnol, 62, 517. Greco O, Folkes LK, Wardman P, Tozer GM, Dachs GU.. (2000). Development of a novel enzyme/prodrug combination for gene therapy of cancer: horseradish peroxidase/indole-3-acetic acid. Cancer Gene Ther, 7, 1414. van Gijlswijk RP, van de Corput MP, Bezrookove V, Wiegant J, Tanke HJ, Raap AK.. (2000). Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization. Histochem Cell Biol, 113, 175. Vianello F, Zennaro L, Di Paolo ML, Rigo A, Malacarne C, Scarpa M.. (2000). Preparation, morphological characterization, and activity of thin films of horseradish peroxidase. Biotechnol Bioeng, 68, 488. Dudkin EA, Gruberg ER.. (1999). Relative number of cells projecting from contralateral and ipsilateral nucleus isthmi to loci in the optic tectum is dependent on visuotopic location: horseradish peroxidase study in the leopard frog. J Comp Neurol, 414, 212. Rota C, Chignell CF, Mason RP.. (1999). Evidence for free radical formation during the oxidation of 2'-7'-dichlorofluorescin to the fluorescent dye 2'-7'-dichlorofluorescein by horseradish peroxidase: possible implications for oxidative stress measurements. Free Radic Biol Med, 27, 873. Schonhuber W, Zarda B, Eix S, Rippka R, Herdman M, Ludwig W, Amann R.. (1999). In situ identification of cyanobacteria with horseradish peroxidase-labeled, rRNA-targeted oligonucleotide probes. Appl Environ Microbiol, 65, 1259. Wu TP, Zheng L, Ruan KC.. (1998). Effect of Calcium Ion on Conformation of Horseradish Peroxidase Isoenzyme C. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai), 30, 510. Kressel M.. (1998). Tyramide amplification allows anterograde tracing by horseradish peroxidase-conjugated lectins in conjunction with simultaneous immunohistochemistry. J Histochem Cytochem, 46, 527. Mendez I, Hong M.. (1997). Reconstruction of the striato-nigro-striatal circuitry by simultaneous double dopaminergic grafts: a tracer study using fluorogold and horseradish peroxidase. Brain Res, 778, 194. Seccia M, Albano E, Maggi E, Bellomo G.. (1997). Circulating autoantibodies recognizing peroxidase-oxidized low density lipoprotein. Evidence for new antigenic epitopes formed in vivo independently from lipid peroxidation. Arterioscler Thromb Vasc Biol, 17, 134.
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使用文献 |
Almeida LE Imasato H, Tabak M. (2006). Enzymatic oxidation of dipyridamole in homogeneous and micellar solutions in the horseradish peroxidase-hydrogen peroxide system. Biochim Biophys Acta, 216.
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[注意事項] |
【安衛法】この商品は、労働安全衛生法で定める皮膚等障害化学物質等を含みます。
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メーカー |
品番 |
包装 |
ABD
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11552
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1 KIT [500 Tests]
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※表示価格について
当社在庫 |
なし
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納期目安 |
1週間程度
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法規制 |
安
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保存温度 |
-20℃
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