別品名 |
Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, PPOL, primary and secondary antibody pair, Primary+Secondary pair, matched antibody pair
|
種由来 |
Human
|
適用 |
Western Blot
|
免疫動物 |
Rabbit
|
交差種 |
Human
|
Accession No.(Gene/Protein) |
P09874
|
Gene Symbol |
PARP1
|
形状 |
滅菌済み液状品
|
構成内容 |
●Anti-PARP1 (internal) (RABBIT) Antibody ●Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Peggy SueTM Size Separation Electropherogram of OVCAR 8 lysates in no salt buffer and detected with Anti PARP1 (internal). UV immobilization time: 250 seconds. Protein concentration: 577 ug/mL; 120 s UV immobilization. Primary antibody concentration: 20ug/mL. Primary antibody incubation time: 180 min. Exposure time: 10 seconds. Predicted/observed: ~116 kDa. Image courtesy of Phil Lorenzi at MD Anderson.
OVCAR-8 Wild Type Lysate separated on 2D SDS-PAGE and blotted on PVDF to analyze immunocoverage of PARP1 antibody specific for the autocatalytic domain of PARP1. Primary Antibody: Anti-PARP1 (internal) antibody 1:200 overnight at 4C. Secondary Antibody: Goat anti-rabbit Peroxidase (p/n 611-103-122) at 1:2,000 at RT for 30min. Blocking Buffer: BlockOut (p/n MB-073) for 30min at RT. Predicted/observed: ~110 kDa and pI 9.7. Other spots detected: cleavage products of PARP1.
Western Blot of Rabbit Anti-PARP1 (internal) Antibody. Lane 1: OVCAR8 Wild Type lysate. Lane 2: OVCAR8 PARP1 KO lysate. Load: 5 μg per lane. Primary antibody: PARP1 (internal) antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: ~113kDa endogenous for PARP1. Other band(s): nonspecific ~ 40kDa.
Western Blot of recombinant PARP1 with Rabbit anti-PARP1 (internal) antibody. Lane 1: PARP1-autocatalytic domain recombinant protein. Load: 0.05 ug per lane. Primary antibody: PARP1 (internal) antibody at 1ug/mL for overnight at 4C. Secondary antibody: HRP Gt-a-rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: ~19 kDa for rPARP1 (internal) Other band(s): none.
Western Blot of Rabbit anti-PARP1 antibody. Lane 1: Molecular Weight ladder. Lane 2: OVCAR-8 Wild Type. Lane 3: PARP1-KO. Lane 4: PARP2-KO. Lane 5: PARP3-KO. Lane 6: PARP4-KO Lane 7: PARP5a-KO. Lane 8: PARP5b-KO. Lane 9: PARP6-KO. Lane 10: PARP7-KO. Lane 11: PARP8-KO. Lane 12: PARP9-KO. Lane 13: PARP10-KO. Lane 14: PARP12-KO. Lane 15: PARP13-KO. Lane 16: PARP14-KO. Lane 17: PARP16-KO. Load: 5.0 ug per lane. Primary antibody: PARP1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Goat anti-rabbit Peroxidase secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Blocking Buffer: MB-073 for 30 min at RT. Predicted size: ~113kDa for PARP1. Observed nonspecific ~40kDa.
|
|
Peggy SueTM Size Separation Electropherogram of OVCAR 8 lysates in no salt buffer and detected with Anti PARP1 (internal). UV immobilization time: 250 seconds. Protein concentration: 577 ug/mL; 120 s UV immobilization. Primary antibody concentration: 20ug/mL. Primary antibody incubation time: 180 min. Exposure time: 10 seconds. Predicted/observed: ~116 kDa. Image courtesy of Phil Lorenzi at MD Anderson.
|
|
|
メーカー |
品番 |
包装 |
RKL
|
K-X51
|
1 PACK
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
保存温度 |
-20℃
|
|