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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA891731LA01HU at dilution of 1:100
IHC image of CSB-PA891731LA01HU diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA891731LA01HU diluted at 1:1200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot All lanes: Protein argonaute-2 antibody at 2μg/ml Lane 1: A549 whole cell lysate Lane 2: Jurkats whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Raw264.7 whole cell lysate Lane 6: K562 whole cell lysate Lane 7: Mouse liver tissue Lane 8: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 98, 94 kDa Observed band size: 94 kDa
Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-AgO2 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP891731HU) against the SLC1A5 promoter.
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