種由来 |
Human
|
標識物 |
Unlabeled
|
精製度 |
Ig fraction - Protein A
|
適用 |
Enzyme Linked Immunosorbent Assay
|
免疫動物 |
Rabbit Mono
|
抗体クラス |
IgG
|
クローン |
RM122
|
交差種 |
Human
|
GENE ID |
3497
|
Accession No.(Gene/Protein) |
P01854
|
分子量 |
70 kDa
|
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Sandwich ELISA using RM122 as the capture antibody (25ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
A titer ELISA using RM122. The plate was coated with different amounts of human IgE (from plasma). A serial dilution of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA of human immunoglobulins shows RM122 only reacted to human IgEλ from human myeloma plasma and the IgE from human plasma. No cross reactivity with Human IgG, IgM, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL or 10 ng/mL of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
|
|
Sandwich ELISA using RM122 as the capture antibody (25ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
|
|
|
メーカー |
品番 |
包装 |
REV
|
31-1024-00
|
100 UG [1 mg/mL]
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
保存温度 |
-20℃
|
|