Bovine Serum Albumin 30% Solution  

Bovine Serum Albumin 30% Solution

Internalization of basigin monoclonal antibodies (mAbs) in hCMEC/D3 cells. (a) Representative confocal images of selected basigin mAbs exposed to hCMEC/D3 cells for 10 and 30？min. Basigin mAbs in green and nuclei visualized by Hoechst in blue. The pictures are the maximum projection of the z-stack, and the XZ projection is below the pictures. Scale bar 30？μm. (b) Quantification of intracellular spots using Cellomics Arrayscan after acid stripping and staining. The intensities are normalized to the negative control and plotted as the percentage increase in spot intensity per cell with？±？standard error of the mean (SEM). ？Secondary antibody diluted in PBS with 2% BSA (p/n BSA-30).？Figure 6. PMID: 32884039.

Surface plasmon resonance sensorgrams representing the binding of basigin extracellular domain to captured anti-basigin monoclonal antibodies. The fitted curves used for equilibrium dissociation constant (KD) calculations were obtained using the Langmuir 1:1 binding model in the Biacore S200 evaluation software and plotted in GraphPad Prism (colored in black). (a) bin A, (b) bin D, (c) bin AD, (d) bin B, and (e) bin C.？At the end of each cycle, the sensor surface was regenerated in 3？M MgCl2？for 30？s. 1？×？HBS-P？+？with 1？mg/ml bovine serum albumin (BSA, p/n BSA-30) was used as running buffer for the kinetic analyses. mAb？monoclonal antibody,？RU？response unit. Figure 3. PMID: 32884039.