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Western Blot of ACE2 antibody. Load: human kidney lysate 35 ug per lane. Primary antibody: ACE2 antibody at 2 ug/ml for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 92 kDa, 95 kDa for ACE2. Other band(s): ACE2 splice variants and isoforms.
Immunohistochemistry of ACE2 Antibody. Tissue: paraffin-embedded human kidney tissue. Fixation: formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: Anti-ACE2 at 2μg/ml overnight at 2-8C. Secondary Antibody: Goat anti-rabbit IgG H&L (HRP) at 1:250 secondary. Counter stained with Hematoxylin.
Immunofluorescence of Anti-ACE2 Antibody. Tissue: Caco2 Cells. Fixation: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue). Image showing membrane staining on Caco2 cells.
Immunofluorescence of Anti-ACE2 Antibody. Tissue: Human Kidney Tissue. Fixation: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green).
Immunofluorescence of Anti-ACE2 Antibody. Tissue: Human Lung Tissue. Fixation: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Anti-ACE2 Antibody. Tissue: Mouse Lung Tissue. Fixation: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Anti-ACE2 Antibody. Tissue: Human Testis Tissue. Fixation: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Anti-ACE2 Antibody. Tissue: Rat Lung Tissue. Fixation: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Western Blot of Rabbit anti-ACE2 antibody. Lane 1: Human Kidney Lysate. Lane 2: Human Testis Lysate. Load: 15 ug per lane. Primary antibody: ACE2 antibody 4ug/mL for 1hr at RT. Secondary antibody: Goat anti-Rabbit secondary HRP antibody at 1:10000 dilution. Block: 5% BLOTTO/TBST. Predicted MW: ~93kDa. Observed MW: ~130kDa.
Western Blot of Rabbit anti-ACE2 antibody. Lane 1: Mouse Stomach Lysate. Load: 15 ug per lane. Primary antibody: ACE2 antibody 4ug/mL for 1hr at RT. Secondary antibody: Goat anti-Rabbit secondary HRP antibody at 1:10000 dilution. Block: 5% BLOTTO/TBST. Predicted MW: ~93kDa. Observed MW: ~130kDa.
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