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Immunofluorescence of Anti-ACE2 Antibody.
Tissue: Human Testis Tissue.
Fixation: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary
antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Anti-ACE2 Antibody.
Tissue: Human Kidney Tissue.
Fixation: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary
antibody at 1:500 dilution (green).
Immunofluorescence of Anti-ACE2 Antibody.
Tissue: Human Lung Tissue.
Fixation: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary
antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Anti-ACE2 Antibody.
Tissue: Mouse Lung Tissue.
Fixation: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary
antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Anti-ACE2 Antibody.
Tissue: Rat Lung Tissue.
Fixation: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary
antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Anti-ACE2 Antibody.
Tissue: Caco2 Cells.
Fixation: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary
antibody at 1:500 dilution (green) and DAPI counterstain (blue). Image
showing membrane staining on Caco2 cells.
Immunohistochemistry of ACE2 Antibody.
Tissue: paraffin-embedded human kidney tissue.
Fixation: formaldehyde and blocked with 10% serum for 1 h at RT.
Antigen retrieval: heat mediation with a citrate buffer (pH6).
Primary Antibody: Anti-ACE2 at 2μg/ml overnight at 2-8C.
Secondary Antibody: Goat anti-rabbit IgG H&L (HRP) at 1:250 secondary.
Counter stained with Hematoxylin.
Western Blot of Rabbit anti-ACE2 antibody.
Lane 1: Human Kidney Lysate.
Lane 2: Human Testis Lysate.
Load: 15 μg per lane.
Primary antibody: ACE2 antibody 4μg/mL for 1hr at RT. Secondary antibody: Goat anti-Rabbit secondary HRP antibody at 1:10000 dilution.
Block: 5% BLOTTO/TBST.
Predicted MW: ~93kDa.
Observed MW: ~130kDa.
Western Blot of Rabbit anti-ACE2 antibody.
Lane 1: Mouse Stomach Lysate.
Load: 15 μg per lane. Primary antibody: ACE2 antibody 4μg/mL for 1hr at RT.
Secondary antibody: Goat anti-Rabbit secondary HRP antibody at 1:10000 dilution.
Block: 5% BLOTTO/TBST.
Predicted MW: ~93kDa. Observed MW: ~130kDa.
Western Blot of Rabbit anti-ACE2 antibody.
Lane 1: Rat Brain Lysate.
Load: 15 μg per lane.
Primary antibody: ACE2 antibody 4μg/mL for 1hr at RT.
Secondary antibody: Goat anti-Rabbit secondary HRP antibody at 1:10000 dilution.
Block: 5% BLOTTO/TBST.
Predicted MW: ~93kDa.
Observed MW: ~130kDa.
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