Anti ACE2

Immunofluorescence of Rabbit Anti-ACE2 Antibody.
Tissue: Caco2 cells.
Fixation: 4% PFA.
Primary antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat Anti-rabbit IgG secondary antibody at
1:500 dilution (green) and DAPI counterstain (blue). Image
showing membrane staining on Caco2 cells.

Immunofluorescence of Rabbit Anti-ACE2 Antibody.
Tissue: Human Testis Tissue.
Fixation: 4% PFA.
Primary antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary
antibody at 1:500 dilution (green) and DAPI counterstain (blue).

Immunofluorescence of Rb Anti-ACE2 Antibody.
Tissue: Human Lung Tissue.
Fixation: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary
antibody at 1:500 dilution (green) and DAPI counterstain (blue).

Immunofluorescence of Anti-ACE2 Antibody.
Tissue: Rat Lung Tissue.
Fixation: 4% PFA.
Primary Antibody: Anti-ACE2 at 20μg/mL.
Secondary Antibody: Goat anti-rabbit IgG secondary antibody at
1:500 dilution (green) and DAPI counterstain (blue).

Western Blot of different Rabbit anti-ACE2 antibodies.
Lane 1: Human Kidney Lysate.
Lane 2: Human Spleen Lysate.
Lane 3: Human Testis Lysate.
Lane 4: Human Breast Lysate.
Lane 5: Human Intestine Lysate.
Load: 15 μg per lane.
Primary antibody: ACE2 antibody (600-401-X58, 600-401-G71, 600-401-X59) at 2μg/mL and Beta Actin at 1μg/mL for 1hr at RT.
Secondary antibody: Goat anti-Rabbit secondary HRP antibody.
Block: 5% BLOTTO/TBST.
Predicted MW: ~93kDa.
Observed: ~130kDa.

Western Blot of Rabbit anti-ACE2 Antibody.
Lane 1: Human Kidney Lysate.
Lane 2: Human Spleen Lysate.
Lane 3: Human Testis Lysate.
Lane 4: Human Intestine Lysate.
Loading: 15μg of lysates per lane.
Primary Antibody: Anti-ACE2 at 2μg/mL for 1hr at RT in 5% BLOTTO/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000
dilution.
Predicted MW: ~93kDa.
Observed MW: ~130 kDa.

Western Blot of Anti-ACE2 Antibody.
Lane 1: Mouse Spleen Lysate.
Lane 2: Mouse Stomach Lysate.
Load: 15 μg of lysates per lane.
Primary Antibody: Anti-ACE2 2μg/mL for 1hr at RT in 5% BLOTTO/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugated at 1:10,000.
Predicted MW: ~93kDa.
Observed: ~125kDa.

Western Blot of Rabbit Anti-ACE2 Antibody.
Lane 1: Rat Skin Lysate.
Load: 15 μg of lysates per lane.
Primary Antibody: Anti-ACE2 at 2μg/mL for 1h at RT in 5% BLOTTO/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Predicted MW: ~93kDa.
Observed MW: ~125kDa.

Western Blot of ACE2 antibody.
Lane A: human kidney lysate at 0.5 μg/ml.
Lane B: human kidney lysate at 1 μg/ml.
Lane C: human kidney lysate at 2 μg/ml.
Load: 35 μg per lane.
Primary antibody: ACE2 antibody at designated concentrations for overnight at 4C.
Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block: 5% BLOTTO overnight at 4C.
Predicted MW: 93 kDa.
Observed MW: 95 kDa for ACE2.
Other band(s): ACE2 splice variants and isoforms.

Immunofluorescence Microscopy of ACE2 antibody.
Cell Type: human kidney cells.
Fixation: 0.5% PFA.
Antigen retrieval: not required.
Primary antibody: ACE2 antibody at 20 μg/mL for 1 h at RT.
Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT.
Localization: ACE2 is located in the cell membrane and the cytoplasm.
Staining: ACE2 as red fluorescent signal.