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Immunofluorescence Microscopy of ACE2 antibody. Cell Type: human kidney cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: ACE2 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ACE2 is located in the cell membrane and the cytoplasm. Staining: ACE2 as red fluorescent signal.
Western Blot of Anti-ACE2 Antibody. Lane 1: Mouse Spleen Lysate. Lane 2: Mouse Stomach Lysate. Load: 15 μg of lysates per lane. Primary Antibody: Anti-ACE2 2μg/mL for 1hr at RT in 5% BLOTTO/TBST. Secondary: Goat anti-rabbit IgG HRP conjugated at 1:10,000. Predicted MW: ~93kDa. Observed: ~125kDa.
Western Blot of ACE2 antibody. Lane A: human kidney lysate at 0.5 ug/ml. Lane B: human kidney lysate at 1 ug/ml. Lane C: human kidney lysate at 2 ug/ml. Load: 35 ug per lane. Primary antibody: ACE2 antibody at designated concentrations for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted MW: 93 kDa. Observed MW: 95 kDa for ACE2. Other band(s): ACE2 splice variants and isoforms.
Immunohistochemistry of ACE2 antibody. Tissue: human kidney tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: ACE2 antibody at 2 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ACE2 is located in the cell membrane and the cytoplasm. Staining: ACE2 as precipitated red signal.
Immunofluorescence of Rb Anti-ACE2 Antibody. Tissue: Human Lung Tissue. Fixation: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Anti-ACE2 Antibody. Tissue: Rat Lung Tissue. Fixation: 4% PFA. Primary Antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Immunofluorescence of Rabbit Anti-ACE2 Antibody. Tissue: Caco2 cells. Fixation: 4% PFA. Primary antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat Anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue). Image showing membrane staining on Caco2 cells.
Immunofluorescence of Rabbit Anti-ACE2 Antibody. Tissue: Human Testis Tissue. Fixation: 4% PFA. Primary antibody: Anti-ACE2 at 20ug/mL. Secondary Antibody: Goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI counterstain (blue).
Western Blot of Rabbit Anti-ACE2 Antibody. Lane 1: Rat Skin Lysate. Load: 15 μg of lysates per lane. Primary Antibody: Anti-ACE2 at 2μg/mL for 1h at RT in 5% BLOTTO/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Predicted MW: ~93kDa. Observed MW: ~125kDa.
Western Blot of different Rabbit anti-ACE2 antibodies. Lane 1: Human Kidney Lysate. Lane 2: Human Spleen Lysate. Lane 3: Human Testis Lysate. Lane 4: Human Breast Lysate. Lane 5: Human Intestine Lysate. Load: 15 ug per lane. Primary antibody: ACE2 antibody (600-401-X58, 600-401-G71, 600-401-X59) at 2ug/mL and Beta Actin at 1ug/mL for 1hr at RT. Secondary antibody: Goat anti-Rabbit secondary HRP antibody. Block: 5% BLOTTO/TBST. Predicted MW: ~93kDa. Observed: ~130kDa.
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