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Chromatin Immunoprecipitation Rabbit Anti-H3K27me1 Antibody. ChIP assays were performed using HeLa cells, H3K27me1 Antibody, and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analyzed. IgG (2 μg/IP) was used as negative IP control. qPCR was performed with primers for the promoter and the coding region of the active gene GAPDH used as a negative and a positive control target, respectively. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Dot Blot results of H3K27me1 antibody. Antigens: H3K27me1 and other modifications and unmodified sequences of histone H3 and H4. Load: 100 pmol, 50 pmol, 25 pmol, 5 pmol, 2 pmol, and 0.2 pmol with control. Primary antibody: H3K27me1 antibody at 1:20,000 for 45 min at 4°C. Secondary antibody: anti-rabbit HRP antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO.
ELISA results of H3K27me1 antibody. Antigen: BSA coupled to H3K27me1. Coating amount: 0.1 μg per well. Dilution series: serial. Titer: 1:32,900 H3K27me1 antibody. Substrate: TMB (p/n TMBE-1000).
Western Blot results of Rabbit anti-Histone H3K27me1 antibody. Lane 1: 15 μg of histone extracts from HeLa cells. Primary antibody: Rabbit anti-Histone H3 K27 me2 antibody at 1:1,000. Secondary antibody: Peroxidase anti-rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: TBS-Tween / 5% BLOTTO. Predicted/Observed size: ~15 kDa for Rabbit Histone H3 K27 me2.
Immunofluorescence results of Anti-Histone H3K27me1 Antibody. Tissue: Human osteosarcoma (U2OS) cells. Fixation: 4% formaldehyde for 20 min. Block: PBS/Triton X-100 / 5% normal goat serum. Incubation: Figure A-E: H3K27me1 antibody 1:1,000. Figure B: 2ng/μl H3K27 peptide. Figure C: 2ng/μl H3K27me1 peptide. Figure D: 2ng/μl H3K27me2 peptide. Figure E: 2ng/μl H3K27me3 peptide. Secondary antibody: anti-rabbit Alexa568 secondary antibody at 1:10,000 for 45 min at RT (Left) or DAPI (right). Staining: Histone H3K27me1 antibody as (red) fluorescent signal, DAPI (blue).
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Chromatin Immunoprecipitation Rabbit Anti-H3K27me1 Antibody. ChIP assays were performed using HeLa cells, H3K27me1 Antibody, and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analyzed. IgG (2 μg/IP) was used as negative IP control. qPCR was performed with primers for the promoter and the coding region of the active gene GAPDH used as a negative and a positive control target, respectively. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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| 別品名 |
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/e, Histone H3/f, Histone H3/g, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
Chromatin Immunoprecipitation
Dot Blot
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| 免疫動物 |
Rabbit
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| 抗体クラス |
IgG
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| GENE ID |
3020
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| Accession No.(Gene/Protein) |
NP_002098, P84243
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| Gene Symbol |
HIST1H3A
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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600-401-W54
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50 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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