| 別品名 |
PKR Antibody, PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2
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| 抗原部位 |
C-terminus
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
Mouse
Rat
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| GENE ID |
5610
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| Accession No.(Gene/Protein) |
AAP57628, P19525
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| Gene Symbol |
EIF2AK2
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| 形状 |
滅菌済み液状品
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Anti PKR Antibody. Loading: 15 ug of lysates per lane. Primary Antibodies: PKR (p/n 600 401 DP7) (1 ug/mL), PKR (p/n 600 401 DP8) (1 ug/mL), and beta actin (1 ug/mL), 1hr at RT in 5% NFDM/TBST. Secondary Antibody: Goat anti rabbit IgG HRP conjugate at 1:10,000. Expect: ~62kDa.
Immunohistochemistry of Rabbit Anti-PKR Antibody. Tissue: Mouse Lung. Fixation: paraffin-embedded formaldehyde and blocked with 10% serum for 1 h at RT. Antigen retrieval: heat mediation with a citrate buffer (pH6). Primary Antibody: anti-PKR antibody at 5ug/ml overnight at 4C. Secondary Antibody: goat anti-rabbit IgG H&L (HRP) at 1/250. Counter stained with Hematoxylin.
Immunohistochemistry of PKR antibody. ？ Tissue: ？rat lung tissue. ？ Fixation: ？formalin fixed paraffin embedded. ？ Antigen retrieval: ？not required. Primary antibody: ？PKR antibody at 2.5 ug/mL ？for 1 h at RT. Secondary antibody: ？Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ？PKR is nuclear and cytoplasmic. Staining: ？PKR is stained with toluidine blue.
Immunofluorescence of Rabbit Anti-PKR Antibody. Tissue: Mouse Lung. Fixation: 4% paraformaldehyde-fixed Primary Antibody: PKR at 20μg/mL, Secondary Antibody: goat anti-rabbit IgG antibody at 1/500 dilution (red) and DAPI staining (blue).
Immunofluorescence Microscopy of PKR antibody. ？ Cell Type: rat lung cells. ？ Fixation: ？0.5% PFA.？ Antigen retrieval: ？not required. Primary antibody: ？PKR antibody at 20 ug/mL ？for 1 h at RT. Secondary antibody: ？Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ？PKR is nuclear and cytoplasmic. Staining: ？PKR as red fluorescent signal.
Western Blot of PKR antibody in A431 whole cell lysate. ？ Lane A: ？PKR antibody at 0.5 ug/mL. ？ Lane B: ？PKR antibody at 1 ug/mL. Lane C: ？PKR antibody at 2 ug/mL. ？ Load: ？35 ug per lane. Primary antibody: ？PKR antibody at designated concentrations for overnight at 4C. Secondary antibody: ？ Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: ？5% BLOTTO overnight at 4C. Predicted/Observed size: ？62 kDa, 72 kDa for PKR.
Western Blot of Rabbit Anti-PKR Antibody. Lysates: Human Cell Lines. Loading: 15ug of lysates per lane. Primary Antibody: PKR (1ug/mL), 1hr at RT in 5% NFDM/TBST. Secondary Antibody: Goat anti-rabbit IgG HRP conjugate at 1:10,000. Expect: ~62kDa.
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Western Blot of Anti PKR Antibody. Loading: 15 ug of lysates per lane. Primary Antibodies: PKR (p/n 600 401 DP7) (1 ug/mL), PKR (p/n 600 401 DP8) (1 ug/mL), and beta actin (1 ug/mL), 1hr at RT in 5% NFDM/TBST. Secondary Antibody: Goat anti rabbit IgG HRP conjugate at 1:10,000. Expect: ~62kDa.
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| メーカー |
品番 |
包装 |
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RKL
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600-401-DP7
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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