Anti Caspase-9  

Western Blot of Caspase-9 antibody. Lane 1: HeLa whole cell lysate.. Load: 35 μg per lane. Primary antibody: Caspase-9 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 46.3 kDa, 46 kDa for Caspase-9. Other band(s): Caspase-9 splice variants and isoforms.

Immunofluorescence Microscopy of Caspase-9 antibody. Tissue: K562 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Caspase-9 antibody at 20 μg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-9 is nuclear, mitochondrial, cytoplasmic, and apoptosomal. Staining: Caspase-9 as green fluorescent signal.

Immunocytochemistry of Caspase-9 antibody. Cells: K562. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Caspase-9 antibody at 2 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-9 is nuclear, mitochondrial, cytoplasmic, and apoptosomal. Staining: Caspase-9 as precipitated brown signal with hematoxylin blue nuclear counterstain.