Anti Caspase-4  

Immunofluorescence Microscopy of Caspase-4 antibody. Cell Type: A20 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Caspase-4 antibody at 10 μg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-4 is located in the endoplasmic reticulum, cell membrane, and mitochondrion. Staining: Caspase-4 as green fluorescent signal.

Western Blot of Caspase-4 antibody. Lane A: Caspase-4 antibody at 1 μg/mL. Lane B: Caspase-4 antibody at 2 μg/mL. Load: 35 μg per lane. Primary antibody: Caspase-4 antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 43 kDa, 31 kDa for Caspase-4. Other band(s): Caspase-4 splice variants and isoforms.

Immunohistochemistry of Caspase-4 antibody. Tissue: human spleen tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Caspase-4 antibody at 2 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-4 is located in the endoplasmic reticulum, cell membrane, and mitochondrion. Staining: Caspase-4 is stained with toluidine blue.