| 別品名 |
Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, eIF-2C2, eIF2C 2, PAZ Piwi domain protein, PPD, Protein slicer
|
| 抗原部位 |
a.a.47-859
|
| 種由来 |
Human
|
| 標識物 |
Unlabeled
|
| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
Immuno Fluorescence
Immunoprecipitation
|
| 免疫動物 |
Mouse
|
| 抗体クラス |
IgG1
|
| クローン |
2A8
|
| 交差種 |
Human
Mouse
|
| GENE ID |
27161
|
| Accession No.(Gene/Protein) |
NP_001158095, Q9UKV8
|
| Gene Symbol |
AGO2
|
| 形状 |
滅菌済み液状品
|
| 参考文献 |
Takeda H, Charlier C, Farnir F, Georges M (2010) Demonstrating polymorphic miRNA-mediated gene regulation in vivo:Application to the g+6223G > A mutation of Texel sheep. RNA 16: 1854-1863.
|
| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
|
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Co immunoprecipitation results of Anti Ago antibody. Hela cells were transfected with: pRL TK 4A + pRL TK 4G + pcDNA miR 1 (miR 1 is supposed to target A allele), pRL TK 4A + pRL TK 4G + pcDNA miR 1C (miR 1C is supposed to target G allele), pRL TK 4A + pRL TK 4G + pcDNA miR 377 (miR 377 is supposed to target neither allele). Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Anti Ago antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RTPCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the bottom panel. Results provided by Haruko Takeda (University of Liege, Belgium).
Immunoprecipitation results of Mouse anti-Ago antibody. Load: 10 ul of input (10% of starting material), 10 ul of supernatants after IP (10%), and each IP (~90%). Lane A: 4ug anti-Ago monoclonal antibody. Lane B: 12ug anti-Ago monoclonal antibody. Lane C: 4 ug mouse IgG. Lane D: 12 ug mouse IgG. Primary antibody: Ago antibody at 1:1000 for overnight at 4C. Secondary antibody: anti-mouse HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBS-Tween overnight at 4C.
|
|
|
|
Co immunoprecipitation results of Anti Ago antibody. Hela cells were transfected with: pRL TK 4A + pRL TK 4G + pcDNA miR 1 (miR 1 is supposed to target A allele), pRL TK 4A + pRL TK 4G + pcDNA miR 1C (miR 1C is supposed to target G allele), pRL TK 4A + pRL TK 4G + pcDNA miR 377 (miR 377 is supposed to target neither allele). Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Anti Ago antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RTPCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the bottom panel. Results provided by Haruko Takeda (University of Liege, Belgium).
|
|
|
| メーカー |
品番 |
包装 |
|
RKL
|
200-301-V04
|
50 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 保存温度 |
-２０℃
|
|
