| 別品名 |
DL800, TrueBlot, DL800 TrueBlot ULTRA, DyLightTM 800 TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting, Fluorescent TrueBlot, Ms TrueBlot, Infrared, IR, NIR
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| 種由来 |
Mouse
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| 標識物 |
DyLightTM 800
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Immunohistochemistry
Immuno Fluorescence
Flow Cytometry
Fluorescence based plate assays
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| 免疫動物 |
Rat
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| クローン |
eB144
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| 交差種 |
Mouse
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| 形状 |
凍結乾燥品
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| 参考文献 |
[Pub Med ID]32058219, 30174309, 27064408, 29167339, 29436615
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Mouse TrueBlotR IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti Caspase 7 using Mouse TrueBlotR ULTRA: Anti Mouse Ig HRP (Lane 1) or conventional HRP conjugated anti mouse antibody (Lane 2) note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re immunoblotted using conventional HRP conjugated anti mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Mouse TrueBlotR ULTRA: Anti Mouse Ig HRP detects only native antibody and not denatured heavy and light chains.
Western Blot of Fluorescent TrueBlotR: Anti-Mouse Ig DyLightTM 800. Lane 1: Mouse IgG, Non-reduced. Lane 2: Mouse IgG, Reduced. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescent TrueBlotR: Anti-Mouse Ig DyLightTM 800 at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 160 kDa for Mouse IgG, Non-reduced. Migrates at slightly higher molecular weight than predicted.
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Mouse TrueBlotR IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti Caspase 7 using Mouse TrueBlotR ULTRA: Anti Mouse Ig HRP (Lane 1) or conventional HRP conjugated anti mouse antibody (Lane 2) note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re immunoblotted using conventional HRP conjugated anti mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Mouse TrueBlotR ULTRA: Anti Mouse Ig HRP detects only native antibody and not denatured heavy and light chains.
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| メーカー |
品番 |
包装 |
|
RKL
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18-4517-32
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100 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
毒
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| 保存温度 |
４℃
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