Anti IgG, Fluorescent TrueBlot^(R)  

Western Blot of Fluorescent TrueBlot^(R): Anti-Rabbit IgG DyLight^TM 800. Lane 1: Rabbit IgG, Non-reduced. M: Opal Pre-stained Ladder (p/n MB-210-0500). Lane 2: Rabbit IgG, Reduced. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescent TrueBlot^(R): Anti-Rabbit IgG DyLight^TM 800 at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: ~160 kDa for Rabbit IgG, Non-reduced.

Rabbit TrueBlot^(R) IP / Western Blot: Jurkat cell lysate (0.5 ml of 1x10e7 cells/ml) was incubated with rabbit anti-human Stat1 and immunoprecipitated using Protein G, Protein A and Anti-Rabbit Ig IP Beads. Precipitate from 5x10e5 cells was subjected to electrophoresis, transferred to a PVDF membrane, and Western blotted with anti-Stat1 using Rabbit TrueBlot^(R): Anti-Rabbit IgG HRP

(a. WT mice were fed with a HFD or normal chow (NC) diet for 4.5 months. The heart tissues were harvested for western blot analysis to detect the expression of β1-adrenoceptor, PDE3A, 4A, 4B, 4D and γ-tubulin. Fluorescent TrueBlot^(R): Anti-Mouse IgG DyLight^TM 680 (p/n 18-4417-32) and Fluorescent TrueBlot^(R): Anti-Rabbit IgG DyLight^TM 800 (p/n 18-4516-32) secondary antibodies were used for multi-color detection.？Fig 1. PMID:？39662482.

(B) Genotyping result demonstrates the product of primer F1 and R1 (645？bp in WT alleles) and that of F2 and R2 (505？bp in KO alleles). Heterozygous mice have both alleles. (C) Representative result of immunoprecipitation (IP) followed by western blot (WB) demonstrating loss of the 63 kD？ZKSCAN3 protein in KO heart tissue. Analysis is from 2 WT (WT1, WT2) and 2 knockout (KO1, KO2) mice. Loading control for tubulin expression represents a WB of the input lysates used for immunoprecipitation. (I) Representative western blot of brain lysate of 2 WT and 2？zkscan3−/−？mice demonstrating no difference in LC3B expression. (J) Representative western blot of SQSTM1/p62 and LC3 from WT and KO MEFs under fed (-) and starved (+) conditions. Fig 1. PMID:？28581889

Western blot analysis using a primary rabbit polyclonal antibody to acetylated-lysine residues followed by a secondary Fluorescent TrueBlot^(R): Anti-Rabbit IgG DyLight^TM 800 antibody (p/n 18-4516-32).？(H and I) Western blot analysis of wildtype SMAD7 protein levels with or without acetate or SB204990 (H) or expression of the K64/70A acetylation-defective SMAD7 mutant under these same conditions (I) (n=3 independent experiments). Data represents mean ± SEM, with significance determined by one-way ANOVA with Bonferroni’s multiple comparison test (A, D, E, H, I). * p < 0.05; ** p < 0.01; ns, not significant.Fig 3. PMID:？29429925