Anti PPARGC1A  

Figure 1 WB Validation in Human Cell Lines
Loading: 15 μg of human cell lysate Antibodies: PPARGC1A 7793, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Human Lung
Loading: 15 μg of human lung lysate Antibodies: PPARGC1A 7793, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 WB Validation in Mouse Skin
Loading: 15 μg of mouse skin lysate Antibodies: PPARGC1A 7793, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 WB Validation in Rat Kidney
Loading: 15 μg of rat kidney lysate Antibodies: PPARGC1A 7793, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 5 Immunofluorescence Validation of PPARGC1A in MCF7 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling PPARGC1A with 7793 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 6 Immunofluorescence Validation of PPARGC1A in HELA Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PPARGC1A with 7793 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Figure 7 Immunohistochemistry Validation of PPARGC1A in Human Colon Carcinoma Tissue
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PPARGC1A antibody (7793) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 8 Immunohistochemistry Validation of PPARGC1A in Human Pancreas Tissue
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PPARGC1A antibody (7793) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 9 Immunohistochemistry Validation of PPARGC1A in Mouse Stomach Tissue
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PPARGC1A antibody (7793) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 10 Immunohistochemistry Validation of PPARGC1A in Rat Kidney Tissue
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PPARGC1A antibody (7793) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.