| 別品名 |
KEAP1 Antibody: INrf2, KLHL19, INRF2, KIAA0132, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2
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| 抗原部位 |
C-terminus
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| 種由来 |
Human
|
| 標識物 |
Unlabeled
|
| 精製度 |
Affinity Purified
|
| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immuno Fluorescence
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| 免疫動物 |
Rabbit
|
| 抗体クラス |
IgG
|
| 交差種 |
Human
Mouse
Rat
|
| GENE ID |
9817
|
| Accession No.(Gene/Protein) |
NP_987096
|
| Gene Symbol |
KEAP1
|
| 形状 |
液状
|
| 推奨品 |
ポジティブコントロール 品番:1302 - Human Lung Tissue Lysate
|
| その他 |
[Protein GI Number]45269145
[Swiss-Prot No]Q14145
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| 参考文献 |
Zhang DD, Lo SC, Cross JV, et al. Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex. Mol. Cell. Biol. 2004;24:10941-53.
Kobayashi A, Kang MI, Okawa H, et al. Oxidative stress sensor Keap1 functions as an adaptor for Cul3-based E3 ligase to regulate proteasomal degradation of Nrf2. Mol. Cell. Biol. 2004; 24:7130-9.
Jiang J, Mo ZC, Yin K, et al. Epigallocatechin-3-gallate prevents TNF-α-induced NF-kappaB activation thereby upregulating ABCA1 via the Nrf2/Keap1 pathway in macrophage foam cells. Int. J. Mol. Med. 2012; 29:946-56.
Devling TW, Lindsay CD, McLellan LI, et al. Utility of siRNA against Keap1 as a strategy to stimulate a cancer chemopreventive phenotype. Proc. Natl. Acad. Sci. USA 2005; 102: 7280-5A.
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※サムネイル画像をクリックすると拡大画像が表示されます。
Figure 1 Western Blot Validation of KEAP1 in Human Lung
Loading: 15 μg of lysates per lane.Antibodies: KEAP1 7045 (A: 1 μg/mL; B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: KEAP1 7045 (2 μg/mL), KEAP1 22-002 (2 μg/mL, and GAPHDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 3 KO Validation in HeLa cells
Loading: 10 μg of HeLa WT cell lysates or KEAP1 KO cell lysates. Antibodies: KEAP1 7045 (2 μg/mL) and beta-actin 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 4 Immunofluorescence Validation of KEAP1 in Human Lung
Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling KEAP1 with 7045 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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|
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Figure 1 Western Blot Validation of KEAP1 in Human Lung
Loading: 15 μg of lysates per lane.Antibodies: KEAP1 7045 (A: 1 μg/mL; B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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|
| メーカー |
品番 |
包装 |
|
PSC
|
7045
|
20 UG
[1 mg/mL]
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※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
3週間程度
|
| 保存温度 |
-２０℃
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