別品名 |
IL-33 Antibody: DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26, NFHEV, Interleukin-33, Interleukin-1 family member 11, IL-33
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot IHC paraffin embedding section Enzyme Linked Immunosorbent Assay Immuno Fluorescence
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免疫動物 |
Rabbit
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抗体クラス |
IgG
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交差種 |
Human Mouse Rat
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GENE ID |
90865
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Accession No.(Gene/Protein) |
NP_254274
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Gene Symbol |
IL33
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形状 |
液状
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推奨品 |
ポジティブコントロール 品番:1369 - Human Lymph Node Tissue Lysate
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その他 |
[Protein GI Number]15559209 [Swiss-Prot No]O95760
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参考文献 |
Schmitz J, Owyang A, Oldham E, et al. IL-33, and interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 2005; 23:479-90. Dinarello CA. Interleukin-18, a proinflammatory cytokine. Eur. Cytokine Netw. 2000; 11:483-6. Brint EK, Xu D, Liu H, et al. ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance. Nat. Immunol. 2004; 5:373-9. Chackerian AA, Oldham ER, Murphy EE, et al. IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol. 2007; 179:2551-5.
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Figure 1 Western Blot Validation of IL-33 in A20 Cells Loading: 15 μg of lysates per lane.Antibodies: IL-33, 4273, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: 1 μg/mLLane B: 2 μg/mL
Figure 2 Western Blot Validation of IL-33 with Human Recombinant Protein Loading: 5ng (1) and 25ng (2) of IL-33 partial human recombinant protein.Antibodies: IL-33, 4273, 1 μg/mL , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Il-33 partial human recombinant protein: 112-270aa, 17.9kD, 36kD band is the dimer.
Figure 3 Western Blot Validation of IL-33 in Human Lymph Node Loading: 15. μg of human lymph node lysateAntibodies: IL-33, 4273, 2 μg/mL , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 4 Western Blot Validation of IL-33 in Human Lung Loading: 15. μg of human lung lysateAntibodies: IL-33, 4273, 2 μg/mL , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 5 Immunofluorescence Validation of IL-33 in Human Lymph Node Immunofluorescent analysis of 4% paraformaldehyde-fixed human lymph node tissue labeling IL-33 with 4273 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Figure 6 Immunohistochemistry Validation of IL-33 in Human Lymph Node Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-33 antibody (4273) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4℃. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 7 Induced Expression Validation of IL-33 in Human Skin Fibroblasts (Ivanov et al., 2010) Protein expression of IL-33 in human skin fibroblasts following alpha-irradiation were detected by anti-IL-33 antibodies. IL-33 levels were increased 1?4 h after α-irradiation.
Figure 8 Regulated Expression Validation of IL-33 in Human Skin Fibroblasts (Ivanov et al., 2010) Protein expression of IL-33 in human skin fibroblasts following alpha-irradiation were detected by anti-IL-33 antibodies. IL-33 levels were increased after α-irradiation, which were inhibited by treatment of PPP.
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Figure 1 Western Blot Validation of IL-33 in A20 Cells Loading: 15 μg of lysates per lane.Antibodies: IL-33, 4273, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: 1 μg/mLLane B: 2 μg/mL
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メーカー |
品番 |
包装 |
PSC
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4273
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20 UG [1 mg/mL]
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※表示価格について
当社在庫 |
なし
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納期目安 |
3週間程度
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保存温度 |
-20℃
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