種由来 |
Human
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由来詳細 |
Kidney carcinoma
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その他 |
[内容・その他(仕様(形状))]凍結細胞 Organism:Homo sapiens (human) Ethnicity:Caucasian Age/Stage:40 years of age Gender:female Tissue:Breast Morphology:epithelial Celltype:infiltrating duct carcinoma Growth Properties:monolayer Description:The MX-1 cell line has been established in vitro from the primary infiltrating duct carcinoma of a 40 year-old female; oestrogen receptors negative. Culture Medium:DMEM:Ham's F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). Subculturing:Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5mlper T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. Note: The cells do not form a confluent monolayer. Subculture when a dense layer of cells is observed macroscopically. Split Ratio:A ratio of 1:2 or maximum 1:3 is recommended Fluid Renewal:every 2 to 3 days Doubling Time:27 hours Freeze Medium:CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) Sterility:Tests for mycoplasma, bacteria and fungi were negative Biosafety Level:1 Tumorigenic:yes, in nude mice DNA Profile (STR):Amelogenin: X, X CSF1PO: 11, 11 D13S317: 11, 12 D16S539: 12, 12 D5S818: 12, 12 D7S820: 11 ,12 THO1: 7, 9 TPOX: 8, 8 vWA: 17, 18 D3S1358: 15, 15 D21S11: 29, 30, 32 D18S51: 12, 16 Penta E: 14, 14 Penta D: 9, 11 D8S1179: 11, 12, 13 FGA: 20, 20
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メーカー |
品番 |
包装 |
CLI
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300107-SF
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1 VIAL
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※表示価格について
販売状況 |
品番変更、サイズ変更
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当社在庫 |
なし
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保存温度 |
液体窒素
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