Organism:Homo sapiens (human)
Age/Stage:40 years of age
Celltype:infiltrating duct carcinoma
Description:The MX-1 cell line has been established in vitro from the primary infiltrating duct carcinoma of a 40 year-old female; oestrogen receptors negative.
Culture Medium:DMEM:Ham's F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400).
Subculturing:Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5mlper T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
Note: The cells do not form a confluent monolayer. Subculture when a dense layer of cells is observed macroscopically.
Split Ratio:A ratio of 1:2 or maximum 1:3 is recommended
Fluid Renewal:every 2 to 3 days
Doubling Time:27 hours
Freeze Medium:CM-1 (CLS order number: 800125, 25ml, 800150, 50ml)
Sterility:Tests for mycoplasma, bacteria and fungi were negative
Tumorigenic:yes, in nude mice
DNA Profile (STR):Amelogenin: X, X
CSF1PO: 11, 11
D13S317: 11, 12
D16S539: 12, 12
D5S818: 12, 12
D7S820: 11 ,12
THO1: 7, 9
TPOX: 8, 8
vWA: 17, 18
D3S1358: 15, 15
D21S11: 29, 30, 32
D18S51: 12, 16
Penta E: 14, 14
Penta D: 9, 11
D8S1179: 11, 12, 13
FGA: 20, 20