別品名 |
HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Flow Cytometry
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免疫動物 |
Mouse
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抗体クラス |
IgG1κ
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クローン |
33A12.G9
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交差種 |
Human
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GENE ID |
3569
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Accession No.(Gene/Protein) |
NP_000591, P05231
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Gene Symbol |
IL6
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形状 |
滅菌済み液状品
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参考文献 |
Novick, D., et al. (1989) Monoclonal antibodies for affinity Purification of IL-6/IFNveta 2 and for neutralization of HGF activity. Hybridoma 8: 561-567. Novick, D., et al. (1992) Enhancement of interleukin 6 cytostatic effect on human breast carcinoma cells by soluble IL-6 receptor from urine and reversion by monoclonal antibody. Cytokine 4 (1): 6-11. Silvera, M.R., et al. (1994) Expression of TGFα isoforms by thy-1+ and Thy-1- pulmonary fibroblast subsets: evidence for TGFα as a regulator of IL-dependent stimulation of IL-6. Lymphokine Cytokine Res 13: 277-285
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Flow Cytometry of Human anti IL 6 antibody 209 301 310. Cells: human PBMC. Stimulation: Figure A: unstimulated; Figure B: 1 ug/mL LPS in a protein transport inhibitor for 5 hours. Staining: (surface) x axis: anti CD14, (intracellular) y axis: anti IL 6.
Anti-Human IL-6 Antibody - Flow Cytometry. Human PBMCs were stimulated with 1ug/mL LPS and a transport inhibitor for 4-5 hours. Cells were then suspended in fixation buffer for 10-12 minutes and vortexed briefly. 1mL of permeabilization buffer was added. 0.5mg of Anti-Human IL-6 Antibody was added (0.125 ug/mL control antibody) and incubated in the dark for 30 minutes. 1:100 of strep/PE was added and incubated for 30 minutes. LPS-stimulated samples were compared to unstimulated cells stained with strep/PE.
Western Blot showing detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20C, Anti-Human IL-6 (MOUSE) Antibody (p/n 209-301-310) was used at 1:1000 in 1% BSA-TTBS over night at 4C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:40,000 for 30 min at 20C and imaged using the Bio-Rad VersaDocR 4000 MP. Band indicates correct 17 kDa molecular weight position expected for Human IL-6.
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Flow Cytometry of Human anti IL 6 antibody 209 301 310. Cells: human PBMC. Stimulation: Figure A: unstimulated; Figure B: 1 ug/mL LPS in a protein transport inhibitor for 5 hours. Staining: (surface) x axis: anti CD14, (intracellular) y axis: anti IL 6.
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メーカー |
品番 |
包装 |
RKL
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209-301-310S
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25 UL
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
-20℃
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