別品名 |
HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
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種由来 |
Human
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標識物 |
Unlabeled
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精製度 |
Purified
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適用 |
Western Blot Immunohistochemistry
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免疫動物 |
Rabbit
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交差種 |
Human
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GENE ID |
3569
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Accession No.(Gene/Protein) |
NP_000591, P05231
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Gene Symbol |
IL6
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形状 |
凍結乾燥品
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参考文献 |
Novick, D., et al. (1989) Monoclonal antibodies for affinity Purification of IL-6/IFNveta 2 and for neutralization of HGF activity. Hybridoma 8: 561-567. Novick, D., et al. (1992) Enhancement of interleukin 6 cytostatic effect on human breast carcinoma cells by soluble IL-6 receptor from urine and reversion by monoclonal antibody. Cytokine 4 (1): 6-11. Silvera, M.R., et al. (1994) Expression of TGFα isoforms by thy-1+ and Thy-1- pulmonary fibroblast subsets: evidence for TGFα as a regulator of IL-dependent stimulation of IL-6. Lymphokine Cytokine Res 13: 277-285
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry of Rabbit Anti IL6 Antibody. Tissue: human lymph nodes at pH9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti IL 6 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: IL 6 is cytoplasmic. Staining: IL 6 as precipitated brown signal (B 20x, B 40x) hematoxylin purple nuclear counterstain. Negative Control (A 20x).
Western blot using Rockland's anti-Human IL6 antibody shows detection of a band ~24 kDa in size corresponding to recombinant human IL6 (arrowhead). Molecular weight markers are also shown. After transfer, the membrane was blocked overnight with 1% BSA in TTBS followed by reaction with primary antibody at a 1:500 dilution. Detection occurred using HRP conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) and FemtoMaxTM Chemiluminescent substrate (p/n FEMTOMAX-110). Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
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Immunohistochemistry of Rabbit Anti IL6 Antibody. Tissue: human lymph nodes at pH9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti IL 6 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: IL 6 is cytoplasmic. Staining: IL 6 as precipitated brown signal (B 20x, B 40x) hematoxylin purple nuclear counterstain. Negative Control (A 20x).
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メーカー |
品番 |
包装 |
RKL
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209-401-310
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100 UG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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